Add MS 56155

FLEMING PAPERS. Vol. L. 16 Feb. 1922-10 June 1923.

ff. ii + 237. 324 x 204mm. Hard red cover. Inside the front cover is a label for ‘W. Isacke, Account Book Maker, 211 Edgeware Road, London’.

Fleming used sections of the book at different times and/or simultaneously, and he also has gone back and re-used sections of the notebook at later dates.

Notebook includes:

f. 5. Unfinished contents list.

Section one: 16 Feb.-April 1922.

ff. 6-35, 39-40, 51, 55. Absorption and action of ‘microzyme’ on cocci

i) Microzymes on cocci

ii) Calculation of absorption

iii) Absorption of tear microzyme with AF cocci

iv) Tears and egg on streptococci

v) Absorption of microzyme

vi) The lytic power of emotional tears

vii) Streptococci on eggs and tears

viii) Serum lysozyme after inoculation (first use of the word lysozyme in these notebooks f. 34)

ix) Pus lysozyme and different treatments

x) Absorption of ‘lysozyme’ in tears (f. 38)

Section two: n.d.

ff. 36-38, 56. Resistance

i) Resistant strains of AF coccus

ii) Resistance of nasal mucus resistant strain AF coccus to other extracts and secretions

iii) Resistant forms of AF coccus second generation (f. 39)

Section three; n.d.

ff. 41-50, 52-53. Bactericidal

i) Experiments examining the effect of lysozyme in tears and albumen on various substances and bacteria– sanjarian, B. coli, Pullonin, Chick. B, Canary Cholera, Avisetieus, staphylococcus, Abortus Bang, B. pestis, P. T. B, Rodets

ii) Experiment involving an extract of brain and Fleming’s extract versus coli, typhoid etc.

iii) Experiment comparing mouth and throat streptococcus with tears, nasal mucus and extract from the tonsils

Section four:

1. f. 72. Intracellular digestion of staphylococci; 11 March 1925.

2. f. 73. Lysozyme content of red blood corpuscles

Section five: 24 June-17 Oct. 1922.

ff. 58-60, 65-70, 73. Eggs and Tears

i) Lysozyme in different eggs

ii) Hen and duck egg white on pathogenic bacteria (on plates)

iii) Experiment testing the inhibitory action of eggs

iv) Experiment where egg albumen was injected into a rabbit intravenously

v) Experiment where egg albumen was injected into a rabbit

vi) Titre of rabbit tears to m. lyso

vii) Effect of egg inhibitory in broth on various bugs

viii) Intravenous inject of egg white into rabbit

ix) Antiseptics on lysozyme in tears

Section six: 10 June 1923, n.d.

ff. 169-171. Blood culture

i) Experiments with artificial blood culture

ii) Blood culture media on staphylococcus blood in slide cells

iii) Mixtures of equal parts of (blood and staphylococcus) and various chemical in slide cells

Section seven:

ff. 183v-192. Entitled ‘continuation from the end of the book’ and involves an experiment examining the effect of alkaloids on bactericidal power of blood to staphylococcus in slide cells

Section eight: n.d.

ff. 116, 206-211v, 214, 225. Lined tubes

i) Experiments examining the bactericidal action of leucocytes in heated and unheated serum

ii) Carbolic acid action on bactericidal power of leucocytes in lined tubes

iii) Antiseptics in lined tubes

iv) Heated and unheated serum in lined tubes

v) Bactericidal AF leucocytes with serum and heated serum on lined tubes

Section nine: 13-27 March 1923.

ff. 203-205, 215-224, 230. Slide Cells

i) Range of experiments involving slide cells and antiseptics

ii) Experiment to find the salt content in slide cells

iii) Effect of salt in bactericidal in lined tubes

iv) Equal parts if AF fresh blood and 1/40 volume staphylococcus w/10 and dilution of alkaloid and slide cells

Section ten: 27 March-12^{April 1923.}

ff. 226-228. Blood

i) Dilution of blood and slide cells

ii) Experiment to examine the effect of chemicals on bactericidal power of blood in slide cells

f. iii. Tests with various eyepieces with various microscopes and micrometers.

includes:

• f. iii Scientific equipment: Notes by A. Fleming on eyepieces for micrometers and microscopes: n.d.
• ff. 6-35, 39-40, 51, 55, 58-60, 64-70 Biochemistry: Experiments by A. Fleming rel. to microzyme: 1921-1922.
• ff. 36-38, 56 Biology: Experiments by A. Fleming rel. to resistance: n.d.
• ff. 41-50, 52-53, 183v-192 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• ff. 54, 169-171, 183v-192, 226-228 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• ff. 54, 58-60, 65-70 Biochemistry: Experiments and notes by A. Fleming rel. to lysozyme: 1921-1948, n.d.
• ff. 58-60, 65-70, 73 Biochemistry: Experiments and notes by A. Fleming rel. to eggs: 1909-1948.
• ff. 72, 183v-192 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• ff. 116, 206-211v, 214, 225 Scientific method: Experiments by A. Fleming using lined tubes: n.d.
• ff. 116, 206-211v, 214, 225 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 116, 206-211v, 214, 225 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• ff. 183-192 Chemistry inorganic: Experiment by A. Fleming rel. to alkaloids: n.d.
• ff. 183v-192, 203-205, 215-224, 226-228, 230 Scientific method: Experiments by A. Fleming using slide cells: 1923-1931.