Add MS 56156

FLEMING PAPERS. Vol. LI. 9 Feb. 1924-25 Feb. 1931.

ff. i + 143. 277 x 219mm. Ring binder with hard, black cover. It was advertised as a ‘scholastic notebook’.

The overall theme of the notebook focuses on antiseptics. The notebook is a ring binder to which paper has been added. As a result of this the dates of the experiments vary greatly and it is difficult to know if Fleming went back to experiments or it is just where pieces of paper were placed later on, as a result there are few defined sections of work.

Notebook includes:

Section one:

1. f. 1. Notes on an experiment using a range of antiseptics. They are written on the back of a letter from A.T.R [Mattieu?] from National Institute for Research in Dairying.

2. f. 3. Effect of carbolic acid on leucocytes in human blood, includes diagrams of slide cells; n.d.

3. f. 8 Experiments with human blood and various concentrations of different chemicals; n.d.

4. f. 26. Summary of experiments to determine inhibition of bactericidal value of different antiseptics on staph in the presence of broth medium and muscle extract; n.d.

5. f. 28. Iodine in bactericidal power of blood to Allen streptococcus; 9 Feb. 1924.

6. f. 28. Tests with blood of a menstrual woman (supposed to have higher bactericidal power); 2 June 1924.

Section two: 10^{Feb., 27 Oct. 1924.}

ff 29. Cocaine

i) On bactericidal power of blood

ii) On HCl and emigration of leucs

Section three: n.d.

ff. 30-36. Leucocytes

i) Sodium salts on leucocytes with Allen streptococcus as indicator

ii) Chlorides on bactericidal power of leucocytes – Allen streptococcus as indicator

Section four: 17-22 May 1924, n.d.

ff. 37, 41-47. Antiseptics

i) On blood in slide cells

ii) In slide cells

iii) Diagrams of the effect of carbolic, picric acid and glycerine on Burns streptococcus, a note on the page reads ‘implant too heavy to show any increase when leucocytes destroyed’.

iv) Royal Society Show – Experiment examining antiseptics in blood including diagrams (of which two, Carbolic and Picric, have been removed)

v) f. 43. Experiment examining the effect of eusol on blood using Burns streptococcus (with Fry’s woman’s menstrual blood), includes diagrams

Section five:

1. ff. 48-49. Picric acid on emigration and killing in slide cells and picric acid on emigration, lysozyme and bactericidal power; 28 May 1924.

2. f. 50. Neo Khasuran on emigration, lysozyme and bactericidal power; 29 May 1924.

3. f. 51. Yadil on Allen streptococcus in slide cells; 2 June 1924.

4. f. 52. Experiment on antiseptics in slide cells for the Royal Society; 17 June 1924.

5. f. 53. Salvarsan products on Allen streptococcus in slide cells; n.d.

Section six: 25 Nov. 1924-2 May 1925.

ff. 54-59. Leucocytes

i) Experiment testing a range of chemicals on leucocytes in slide cells

ii) Experiment examining the effect of Salvarsan products on leucocytes

iii) Experiment examining the effect of carbolic and picric acid on leucocytes and phagocytosis

iv) Experiment examining the effect of Flavine and NAB on leucocytes in 24 hours

Section seven:

1. f. 60. Experiment examining the effect of Rivanol on Allen streptococcus in slide cells; 5-8 Oct. 1924.

2. f. 61. Experiment examining the effect of T.C.P (antiseptic) on blood in slide cells; 1 Nov. 1924.

3. f. 72. Experiment examining the effect of antiseptics in blood, immediately and at interval, found that ‘some of the apparent bactericidal power of blood acts on cocci before antiseptic kills leucocytes in weaker dilutions’; 28 Feb. 1925.

4. f. 74. Experiment examining the effect of picric acid on bactericidal phagocytosis and emigration of equal parts, picric dilution and infected blood; 2 Dec. 1924.

5. f. 75. Slide cells – AF blood and chemicals; 29 Feb. 1924.

6. f. 77. Diagram of effect of HgCl2 on Hensons and Allen streptococcus; n.d.

7. f. 78. Flavine on haemolytic coli and staphylococcus on slides cells; n.d.

8. f. 79. Experiment examining the effect of antiseptics in slide cells with chloramine T; 4 June 1925.

9. f. 80. Experiment examining the effect of antiseptics in blood in slide cells (for Yanks); 3 June 1925.

10. f. 85. Gentian violet on various microbes in slide cells; 12 May 1926.

Section eight: 7-14 May 1926.

ff. 86-87. Dyes

i) On streptococcus, coli in slide cells

ii) Oplochin and rivanol on B. coli in slide cells

Section nine:

1. f. 89. Citrate on bactericidal power in slide cells; 22 June 1926.

Section ten: n.d.

ff. 90-94. HgCl2

i) Bactericidal power of blood on staph and strep

ii) Different microbes in slide cells

iii) Diagrams of slide cells

iv) In blood on staph and strep

Section eleven:

1. ff. 96-97. Antiseptics in blood in slides cells (for AEW show) – infected blood with Allen streptococcus or Henson virulent streptococcus; 9 Dec. 1926.

2. f. 98. KCN on leucocytes in slide cells and on leucocytes and emigration in cells; 18 Dec. 1926.

Section twelve: 3 March 1927-28 Nov. 1928.

ff. 100-104. Monsol

i) In slide cells with AF blood

ii) On Jennis in blood

iii) In leucocytes

Section thirteen:

1. f. 105. HgCl2 on Henson streptococcus (virulent in blood); 2 Nov. 1927.

2. f. 106. Bacteriostatic power of HgCl2 in broth; 26 June 1928.

3. f. 108. Acid and alkaline on leucocytes in slide cells; 3 Dec. 1926.

4. ff. 109-110. Effect of carbolic on slide cell emigration and phagocyism; 15 Feb. 1927.

5. f. 111. Flavine on leucocytes and bugs at intervals (with Craddock); 8 Oct. 1928.

6. f. 112. Invenine and emeline HCl on streptococcus, pyog and blood in slide cells; 17 Nov. 1928.

7. ff. 113-114, 116. Work on mercurochrome; 2 Feb. 1931.

8. f. 115. Rate of killing of staphylococci by 1 in 20,000 flavine in AF de-leucocyted blood; 28 Feb. 1931.

9. f. 117. Intravenous flavine; 14 Jan. 1931.

10. f. 121. Experiment with gentian violet and de-leucocyted blood and slide cells; 25 Sept. 1931.

Section twelve: 23 June 1923, n.d.

ff. 126-138. Tuberculosis

Diagrams of the effect of salt, flavine, picric acid, chloramine T. on the growth of TB in AF blood. Includes a summary of the results.

Links with other notebooks:

56159: Mercurochrome and antiseptics

56223: Work on chloramine T. on the growth of B. sporogenes

56224: HgCl2

includes:

• ff. 1, 3, 26, 28, 37, 41-49, 51-52, 60-61, 72, 74, 77-80, 85, 90-94, 96-97, 100-115, 117, 121 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• ff. 3, 30-36, 54-59, 98, 121 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 3, 52, 75, 79, 89, 98, 108, 121 Scientific method: Experiments by A. Fleming using slide cells: 1923-1931.
• ff. 3, 8, 72, 80, 121 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• f. 26 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• ff. 26, 115 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• f. 29 Pharmacology narcotic drugs: Experiments by A. Fleming rel. to cocaine: 1924.
• ff. 48-49, 74 Pharmacology antiseptics: Experiments by A. Fleming rel. to picric acid: 1917-1924.
• ff. 51, 53, 60, 86-87, 96-97, 112 Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• f. 53 Pharmacology: Experiment by A. Fleming rel. to salvarsan: n.d.
• ff. 77, 90-94, 105-106 Pharmacology antiseptics: Experiments by A. Fleming rel. to HgCl2 (mercuric chloride): 1917-1928, n.d.
• ff. 79, 126-135, 137-138 Pharmacology antiseptics: Experiments by A. Fleming rel. to chloramine T: 1925.
• ff. 85, 121 Pharmacology antiseptics: Experiments by A. Fleming rel. to gentian violet: 1927-1942.
• ff. 86-87 Chemical compounds: Experiments by A. Fleming using dyes: 1926-1932.
• ff. 100-104 Pharmacology antiseptics: Experiments by A. Fleming rel. to monsol: 1927-1931.
• ff. 113-114, 116 Pharmacology antiseptics: Experiments by A. Fleming rel. to mercurochrome: 1927-1931, n.d.
• ff. 126-138 Pathology infectious diseases: Experiments by A. Fleming rel. to tuberculosis: 1902-1923, n.d.