Add MS 56157

FLEMING PAPERS. Vol. LII. 10 Feb. 1924-15 Oct.1926.

ff. ii + 70. 276 x 226mm. Ring binder with hard, black cover. It was advertised as a ‘scholastic notebook’.

Label on the spine has ‘Centrifuged Phagocytosis’ on it and other words which have been crossed out.

This notebook has been divided into named sections. However, as it is a ring binder it is difficult to know if the papers are in the correct order or if sheets have been added later on.

Notebook includes:

Section one: 8 Feb.-2 May 1924.

ff. 1-6. Children – Bactericidal and Phagocytosis

i) Experiment examining phagocytosis and children

ii) Experiment examining phagocytosis and killing in infant blood

iii) Child’s blood bactericidal in slide cells

iv) Diagram of normal blood and blood infected with mastron septicaemia

Section two: 20^{Jan. 1925-19 June 1926.}

ff. 7-12. Granules Leucocytes

i) Notes on a septic uterus

ii) Notes on a subphrenic abscess

iii) Experiment with a rabbit which has been given Le Cinnate intravenously and comparison made of samples of blood taken before and after the injection

iv) Experiment to examine AF blood two days after the onset of a cold

v) Notes on leucocytic dendrils. Took AF defibrinated blood, left for half an hour and observations made on movement, phagocytosis and staining, includes diagrams

Section three: 10 Sept. 1925-10 Jan. 1927.

ff. 13-35. Centrifuged Phagocytosis

i) Experiment examining ordinary opsonic versus centrifuged pseudo phagocytosis

ii) Diagram of distribution of leucocytes corpuscles and bugs on opsonic slide

iii) Experiment of Pseudo phagocytose – the effect of time of centrifuging on phagocytosis, including diagrams

iv) Centrifuged opsonics

I – Rate of opsonisation

II – Time of centrifuging on phagocytosis

v) Centrifuged opsonics

I – Effect of different times of centrifuging on opsonised and non opsonised bugs

II – Rate of opsonisation at 37˚C and 18˚C

vi) Experiment examining the effect of time of centrifuging with fully opsonised staphylococcus

vii) Experiment testing staphylococcus opsonised half an hour before phagocytosis

viii) Experiment examining the influence of temperature on phagocytosis

ix) Time of incubating or centrifuging with fully opsonised bugs (coli)

x) Temperature on phagocytosis

xi) Opsonics centrifuged and incubated

xii) Diluted serum opsonics, incubated and centrifuged

xiii) Rate of opsonisation at 16/17˚C (serum and serum/10)

xiv) Opsonics centrifuged and incubated and dilution of serum

xv) Amount of phagocytosis that occurs with different opsonic methods and dilution of serum with heated serum

xvi) Experiment to examine whether centrifuging does increase rate of opsonisation

Section four: 2 Dec. 1925.

ff. 36-37. Removal of leucocytes

Diagram and instructions on how to de-leucocyte blood using cotton wool

Section five: 2 Feb.-15 Oct. 1926.

ff. 38-67. Opsonics

i) Experiments to examine opsonics different ways

ii) Experiment examining the rate of opsonisation at 18˚C on staphylococcus

iii) Experiment examining the rate of opsonics on tuberculosis at 18˚C and tuberculosis and AF mixed, results include graph

iv) Testing AF septicaemia (endocarditic) corpuscles in different ways

v) Experiment to see whether partial or complete opsonisation varies after a vaccine

vi) Different bloods opsonised over short and long periods and at 18˚C and 37˚C, results include a graph

vii) Emphysema blood at 37˚C and 18˚C

viii) Experiment examining the rate of destruction of opsonisation at 60˚C

ix) Opsonisation at 18˚C - complete one hour at 3 (done by RMF (Fry?)

x) Rate of opsonisation coli at 37˚C (done by RMF)

xi) Rate of opsonisation on tuberculosis at 37˚C

xii) Experiment testing opsonisation at 19˚C and 37˚C

xiii) AF serum and staphylococcus after 1 hour 17˚C and 38˚C

xiv) Experiment examining centrifuged and incubated opsonics with heated serum

Links with other notebooks:

56158: Phagocytosis, centrifuging and the removal of leucocytes from blood

56160: Phagocytosis and bactericidal power of blood

56161: Killing and phagocytosis

56163: Centrifuged phagocytosis

includes:

• ff. 1-6 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• ff. 1-6, 13-35 Cell biology: Experiments by A. Fleming rel. to phagocytosis: 1902-1942.
• ff. 7-12, 36-37 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 13-35, 38-67 Cell biology: Experiments by A. Fleming rel. to corpuscles: 1927, n.d.
• ff. 38-67 Bacteriology: Notes and experiments by A. Fleming rel. to opsonics: 1902-1926.