Add MS 56159

FLEMING PAPERS. Vol. LIV. 1 April 1924-16 March 1932.

ff. iii + 87. 276 x 221mm. Ring binder with hard, black cover. It was advertised as a ‘scholastic notebook’.

On the spine of the notebook, the label has been removed but the word ‘blood’ is still visible.

Label inside the cover for ‘W. Isacke, Manufacturing Stationer, Printer… and Account Book maker, 133 Praed St., London.’

This notebook has been divided into sections, one of which is named. However, as it is a ring binder it is difficult to know if the papers are in the correct order or if sheets have been added later on.

Notebook includes:

Section one: 1 April 1924-29 April 1926.

ff. 6-16. Blood Culture

i) Artificial blood culture

ii) AF blood unclotted and planted in bulk with staphylococcus (in slide cells)

iii) Blood culture media – to see what substances prevent clotting (including diagrams of slide cells)

iv) Artificial blood culture – experiment with Am2S04, MgSO4 on streptococcus pyog in slide cells, includes coloured diagrams of the slide cells

v) Blood culture – Edwardson

vi) Blood culture media – slide cells and various streptococcus, experiment has a note written over it ‘no good – pract. no growth’

Section two: 11 June-9 Sept. 1926.

ff. 17-20. Heparin

i) Experiment with heparin and its effect on clotting including test with a rabbit and blood from Fleming’s finger

ii) Heparin and phagocytosis

iii) Heparin on clotting of AF blood – new lot of heparin

Section three: 22 Jan.-3 Feb. 1927.

ff. 21-28. Antiseptics

i) Gentian violet on bacteriosis in blood

ii) Bacteriosis in different mediums – de-leucocyted blood, serum and broth. Tests with flavine, Gentian violet, HgCl2 and picric acid

iii) Bacteriosis by Gentian violet in broth

iv) Flavine bacteriosis and diagrams of slide cells – Gentian noted absorption by paper, includes coloured diagrams of slide cells

Section four:

1. f. 29. Comparative affinity of flavine and carbolic for microbes and filter paper; 10-11 April 1927.

2. f. 30 De-leucocytes (AF) blood and 1/10 volume bugs, 25cmm and chemical 25cm in slide cells; 31 Jan. 1927.

3. f. 31. Experiment using mercurochrome on allen I slide cells; n.d.

Section five: 31 Dec. 1926-20 Oct. 1927

ff. 33-44, 49-75, 80. HgCl2

i) Table with results of experiments with HgCl2 – dilutions of HgCl2 and AF blood infected with bugs

ii) HgCl2 on coli including colour diagrams of slide cells

iii) HgCl2 in slide cells in various bacteria

iv) HgCl2 explanting

v) HgCl2 on Hensons streptococcus

vi) Hgcl2 on leucocytes

vii) HgCl2 on Todd’s movies

viii) HgCl2 on heparinised blood to see if effect is the same as on defibrinated blood

ix) Intravenous injections HgCl2 into guinea pigs

x) HgCl2 on hemo streptococcus in slide cells

xi) Bacteriostasis HgCl2 in different media (for Lister Centenary)

xii) HgCl2 on bactericidal to streptococcus (Needhams) in their slide cells

xiii) HgCl2 blood and de-leucocyted blood in movies

xiv) HgCl2 on leucocytes – morphology

xv) HgCl2 on blood – does it only affect bactericidal power to virulent streptococcus?

xvi) HgCl2 on Hares virulent streptococcus (Edwardson)

xvii) Blood on pneumo, HgCl2 on pneumo

Section six: 21 Oct. 1927, n.d.

ff. 1, 31, 76-78. Mercurochrome

i) On Allen I slide cells

ii) On streptococcus (puerperal from Hare) in slide cells

iii) On the killing power of blood

iv) On bactericidal power

Section six: 8-16 March 1932.

ff. 82-86. Novaseptic (The Perfect Germicide)

Experiments with staphylococcus and other bacteria. A booklet with directions for use of the antiseptic has been attached to a page.

Links with other notebooks:

56156: Mercurochrome and antiseptics, possibly developmental work.

56164: Loose link on work on blood culture

56169: Novaseptic

56224: HgCl2

includes:

• ff. 1, 31, 76-78 Pharmacology antiseptics: Experiments by A. Fleming rel. to mercurochrome: 1927-1931, n.d.
• ff. 6-16 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• ff. 17-20 Biochemistry: Experiments by A. Fleming rel. to heparin, 11 June-9 Sept: 1926.
• ff. 21-28 Pharmacology antiseptics: Experiments by A. Fleming rel. to gentian violet: 1927-1942.
• ff. 21-29, 82-86 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• f. 30 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 33-44, 49-75, 80 Pharmacology antiseptics: Experiments by A. Fleming rel. to HgCl2 (mercuric chloride): 1917-1928, n.d.
• ff. 82-86 Pharmacology antiseptics: Experiments by A. Fleming rel. to 'novaseptic': 1932.