Add MS 56160

FLEMING PAPERS. Vol. LV. 8 May 1924-28 June 1932.

ff. ii + 60. 276 x 224mm. Ring binder with hard, black cover. It was advertised as a ‘scholastic notebook’.

Label on the spine of a notebook has ‘Selective Media’ written on it.

Label inside the cover for ‘W. Isacke, Manufacturing Stationer, Printer… and Account Book maker, 133 Praed St., London.’

This notebook has been divided into four sections, the first of which is unnamed. However, as it is a ring binder it is difficult to know if the papers are in the correct order. The notebook seems to have originally housed plain paper and this has been supplemented with other pieces of plain and lined paper.

Notebook includes:

Section one:

1. f. 1. Experiment with various concentrations of chemicals; 16 July 1930.

2. f. 4. Experiment examining phagocytosis and bactericidal power – found washed corpuscles and heated serum, no bactericidal power; 8 May 1924.

3. f. 6. Experiment lined tubes and washed dilutions (copied from other book), note written in red pen ‘cannot explain these results’; n.d.

4. f. 7. Experiment testing the bactericidal power of sensitive people; n. d.

5. f. 11. Slide cells made with bugs as under 2.5cm; 19 May 1924.

6. f. 12. Experiment involving 2% salt and picric acid in blood; 16 July 1924.

7. f. 13. Experiment examining the killing potential of reconstituted blood and washed cells; 12 March 1925.

Section two: 13-20 May 1927.

ff. 19-23. Walsh’s Citrate and Clotting Bactericidal

i) Experiment to see if citrate acid is added to blood at intervals before clotting occurs, what effect it has on bactericidal power

ii) Citrate added to blood at intervals before clotting – influence on bactericidal power

iii) Citrate at intervals after clotting – influence on bactericidal power

iv) Chiastic acid and defibrinated blood, found:-

a. Reconstituted blood much better than original citrated blood

b. Citrated plasma not so good as defibrinated blood

c. Citrated corpuscles better then defibrinated corpuscles

d. Best killing with defibrinated blood

Section three: 15 Feb. 1929-29 May 1931.

ff. 24-50. Selective Media – Ethylamine, CuS04, Dyes, Phenylhydrazine etc

i) Selective dyes – experiment using dilutions made in broth and then

staphylococcus and coli planted

ii) Selective cultures mouth – 1/10 of saturated solution in fildes broth

and infected with bacteria from AF mouth and planted in boiled blood agar

iii) Selective media CuS04 – dilutions made in broth, equal quantities

of infected broth put in small test tubes and incubated for 24 hours

iv) Experiments with Hancock faeces and glucose broth, alcohol,

Ethylamine, CuS04 and tartar emetic

v) CuSO4 Jean faeces planted in various strengths of CuS04 in

glucose broth grown anaerobically

vi) Seacones faeces in glucose broth and 35cm ethylamine

Boil’ faeces and drop suspension in broth to 1cc of mixtures of dialysed iron, ethylamine, CuS04, tartar emetic, Ethyl acetate and alcohol

vii) Ethylamine on chromogenesis of staphylococcus

vii) Ethylamine – experiments with dilutions of it in glucose broth and

put in small tubes and planted with various microbes

ix) Experiment examining the effect of ethylamine on the pigment

formation of pyocyaneus and ethylamine on prodigiosin

x) Walsh’s fatty acid – inhibitory power of microbic growth in agar

xi) Inhibition of coli by ethylamine – tests ethylamine, CuSO4, NaOH

and acid fuschin

xii) Development of staphylococcus aureus in faeces around CuS04

xiii) ‘Scott’ faeces spread thickly on agar plate and tests carried out with

various strengths of ethylamine, includes diagrams of the results

xiv) Ethylamine on coliforms – diagram with lines representing actual

distance of growth

xv) Phenylhydrazine HCl on faecal cultures – drops of dilutions on

agar and blood agar plates

xvi) Diagram of the effect of amines on staphylococcus

xvi) Chemical inhibition of muscid coli, streptococcus (virulent) and

(mouth) staphylococcus

xiv) Walsh’s emulsion – 10% olive oil and 1.2 % iodine, tests with AF defibrinated and staphylococcus

xix) SuCl2 on various microbes – diagram of agar plates results

xx) Phenylhydrazine inhibition of coli on blood plate

Section four: 2 Feb.-20 June 1932.

ff. 51-60. Tellurite

i) Experiments with mixture of Dunlop’s coli and Dunlop’s step, plus two drops pf K. Tellurite, same experiment using M. Ruth faeces, Waldron faeces and Lyle faeces

ii) Experiment examining the effect of tellurite on haemolytic streptococcus

iii) Tellurite inhibitions – experiments with agar mixed tellurite with in various strengths in plates onto which various microbes were planted

Section five:

1. f. 61. Notes from Topley and Wilson [italics]The Principles of Bacteriology and Immunology[/italics], p. 109, volume 1; n.d.

Links with work in other notebooks:

56157: Phagocytosis and bactericidal power of blood

56161: Killing power of blood

56164: Walsh

56166: Sample taken from ‘Scott’

56169 and 56171: Work on tellurite (although dates differ)

includes:

• f. 4 Cell biology: Experiments by A. Fleming rel. to phagocytosis: 1902-1942.
• ff. 4, 19-23 Cell biology: Experiments by A. Fleming rel. to corpuscles: 1927, n.d.
• ff. 4, 7, 19-23 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• f. 6 Scientific method: Experiments by A. Fleming using lined tubes: n.d.
• f. 6 Chemical process: Experiments by A. Fleming rel. to dilution: [1909]-1921, n.d.
• f. 11 Scientific method: Experiments by A. Fleming using slide cells: 1923-1931.
• f. 12 Pharmacology antiseptics: Experiments by A. Fleming rel. to picric acid: 1917-1924.
• f. 12 Chemical compounds: Experiments by A. Fleming rel. to salt: 1902-1925, n.d.
• ff. 13, 19-23 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• ff. 19-23 Biochemistry: Experiments by A. Fleming rel. to Walsh's citrate, 13-20 May: 1927.
• ff. 24-50 Chemical compounds: Experiments by A. Fleming rel. to ethylamine: 1929-1931.
• ff. 24-50 Chemical compounds: Experiments by A. Fleming rel. to CuSO4: 1929-1931.
• ff. 24-50 Chemical compounds: Experiments by A. Fleming rel. to phenylhydrazine: 1929-1931.
• ff. 24-50 Microbiology: Experiments by A. Fleming rel. to selective media: 1929-1931.
• ff. 24-50 Chemical compounds: Experiments by A. Fleming using dyes: 1926-1932.
• ff. 51-60 Pharmacology antiseptics: Experiments by A. Fleming rel. to tellurite: 1932-1937, n.d.