Add MS 56162

FLEMING PAPERS. LVII. 10 Oct. 1924-20 Jan.1932.

ff. ii + 93. 277 x 218mm. Front and back hard, black covers of a ring binder, spine is missing. It was advertised as a ‘scholastic notebook’.

A section of the spine with the label survives, it has ‘Immunisation in Vitro’ written on it.

Notebook contains the discovery of penicillin.

This notebook has been divided into four sections. The notebook seems to have originally housed plain paper and this has been supplemented with other pieces of plain and lined paper. As there is no spine it is unclear whether notes are in the right order or whether they have been altered at a later date.

Notebook includes:

Section one:

ff. 1-2. Two photographs of Fleming’s report card for his medical training

Section two: 13-23 Oct. 1924.

ff. 3-5. Immunisation in vitro

i) Experiments with fresh and incubated leucocytes

ii) Experiment with de-leucocyted blood and leucocytes

iii) Experiment with faeces filtrate

Section Two: 12 May-15 Nov. 1926.

ff. 6-9. [Bacteria/ bactericidal] slide

i) Experiment with haemolytic B. coli in slide cells

ii) Experiment with haemolytic coli in slide cells in salted blood

iii) Experiment with different streptococcus in slide cells

iv) Experiment to see if killing of staphylococcus various with thickness of the slide cell

Section Three: n.d

ff. 10-14. Worms (penicillin)

i) Experiment with different bugs in slide cells

ii) Diagram of the growth of staphylococcus in cells

iii) Experiment examining the rate of emigration of leucocytes from worms

Section four: 30 Oct. 1928.

ff. 15-16. Discovery of Penicillin

Description of a staphylococcus inhibiting mould, including diagrams

Section five: 22 Nov. 1928-17 Jan. 1929.

ff. 17-39. Experiments with the new ‘mould’

i) Experiments with different bacteria including diagram of results

ii) Inhibitory action of ‘mould’ broth of staphylococcus in blood

iii) Inhibition by moulds, including diagram of plate

iv) Mould inhibition and imbedded agar and mould filtrate streaked across bugs

v) Mould planted in broth; 30 Nov.

vi) Mould inhibitions – different penicillin from milk

vii) Craddock – inhibitions streptococci by mould filtrate

viii) Mould filtrate on Pfeiffer including diagram

ix) Mould filtrate antiseptic power of Craddock’s autrium

x) Conditions of optimum growth of inhibiting mould

xi) Experiments autoclaving the mould filtrate

Section six: 29 Jan.-9 March 1929.

ff. 40-41, 43-48, 51, 55, 59. Inhibitory action

i) Mould inhibitions – penicillin 12

ii) Isolation Pfeiffer by mould inhibition

iii) Formation of inhibitory substance in mould juice

iv) Disappearance of inhibitory substance in penicillin on the bench

Section seven: 29 Jan.-16 Feb. 1929.

ff. 42, 49-50, 57, 67. Nasal mucus and sputum

i) Sputum and nasal mucus in mould juice

ii) Mould juice and Craddock nasal mucus

iii) AF sputum – diagram of results with explanations

iv) Diagram of experiment using Craddock’s nasal mucus and six drops of mould filtrate on an agar plate

Section eight:

1. f. 64. Methylene blue on mould; 28 May 1929.

2. f. 66. Notes from article by Dr Bocchia – Cent F [Balit] 909 l. 220 ‘Die Pyocyanase’, including notes on inhibition in broth and reaction with various bacteria.

3. ff. 68-70. Experiment testing inhibition of methyl violet, blue and penicillin; 10 Oct. 1929.

4. ff. 71-73. Experiments with new inhibiting moulds, pink and green; 24 Sept.-1 Oct. 1929.

5. f. 74. Wellesly sputum spread over a plate and tested with drops of methyl violet, blue and penicillin; 15 Oct. 1929.

6. ff. 78-80. Experiments with old penicillin; 27 May-22 June 1931.

7. f. 82. Penicillin on haemolytic power of streptococcus; 15 July 1931.

8. f. 83. Penicillin on anaerobes; 14 July 1931.

9. f. 86. Reaction of penicillin on keeping properties; 18 Sept. 1931.

10. ff. 87-88. New moulds; 6 Dec. 1931-20 Jan. 1932.

11. f. 91. Diagram of the reaction of various bacteria with crystal violet, aniline blue and penicillin on boiled blood agar; 18 Oct. 1929.

Link with other notebooks:

56158: Loose link on work on immunity in vitro

56166: Loose connection between patient ‘Wellesley’ and experiment with methyl violet and penicillin

56167: Penicillin, in particular Pfeiffer’s

56169: Penicillin and moulds

includes:

• ff. 1-2 Art. Photographs: Sir Alexander Fleming, bacteriologist: A. Fleming's medical training report card: [1908].
• ff. 3-5 Immunology: Experiments by A. Fleming rel. to immunisation in vitro: 1924-1925.
• ff. 6-14 Scientific method: Experiments by A. Fleming using slide cells: 1923-1931.
• ff. 6-9 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• ff. 10-14 Microbiology penicillin: Experiments by A. Fleming rel. to worms: n.d.
• ff. 15-39, 40-41, 43-51, 55, 57-59, 67, 80-83, 86, 91 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 17-39, 40-41, 43-48, 51, 55, 59, 66, 68-73 Chemical process: Experiments by A. Fleming rel. to inhibition: 1917-1942.
• ff. 17-39, 40-41, 44-51, 55, 57, 59, 64, 71-73, 78-80, 87-88 Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• ff. 42, 49-50, 55, 57, 65, 67 Physiology: Experiments by A. Fleming using nasal mucus and sputum: 1929.
• ff. 42, 49-50, 55, 57, 67 Stuart Craddock, physician: Microbiology penicillin: Experiments by A. Fleming using Craddock's nasal mucus: 1929.
• f. 82 Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• f. 82 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• f. 91 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.