Add MS 56168

FLEMING PAPERS. Vol. LXIII. 16 Nov. 1930-8 June 1932.

ff. iii + 98. 276 x 223mm. Ring binder with hard, black cover. It was advertised as a ‘students ring book’.

It is actually being used back to front and label inside the back is for ‘Isacke, Printer, Stationer, Account Book Maker. 133, Praed Street, W.2.’

As the notebook is a ring binder it is difficult to know if the papers are in the correct order. The notebook seems to have originally housed plain paper and this has been supplemented with other pieces of plain and lined paper. Label on the spine of the notebook has ‘Mycolysis’ written on it.

Notebook includes:

Section one:

1. ff. 2-5. Experiments with monsol, includes diagrams; 15 July 1931.

2. f. 7. Notes on vaccines; n.d.

3. f. 10. Diagram of filtration medium, n.d.

Section two: 16 Nov.-4 Dec. 1930.

ff. 15, 17-68. Andre Gratia

i) Notes possibly for an article related to the work of Andre Gratia on lysis, n.d.

ii) Notes from an articles by Gratia and Sara Dash in CR Society Biology 1924 XCI 1442 and 1925 XCII 461, on Streptothrix lysing bacteria

iii) Diagram anticoli versus typhoid

iv) Various experiments with anticoli and antistaph

v) Experiment examining phage using anticoli 7, includes a diagram

vi) Experiment examining the lysis of B. coli by broth cultures of anticoli and antistaph

vii) Rate of lysis by ‘anticoli 8’ with and without glucose, includes diagrams of plates

viii) Notes from A. Gratia and Sara Dash’s articles in CR Society Biology 1924 XCI 1442 and 1925 XCII 461, 451

ix) Notes from an article by A. Gratia and J. Alexander on ‘Mycolysis’ in CR Society Biology 1931 CVI 1288

x) Gratia’s lysis – tests staphylococcus and coli

xi) Diagram of a plate with ‘streptothrix’ colony. It has been left for ten days, staphylococcus and coli added and left for another 24 hours later. Noted that results indicate ‘apparent inhibition of growth’

xii) Gratia lysis – test the potency of ‘streptothrix’ on coli. Anti-staph and coli ‘streptothrix’ grown in broth flask at 22°C for three months, dilutions made and various bacteria added

xiii) Experiments with mycolysis, including whether it inhibits living coli. Results include diagrams of plates

xiv) Patient notes

a. Wallace – rheumatoid arthritis

b. Tinker – oral ulcers

c. Whin – chronic erysipeloid condition of the face

xv) Inoculation of rabbits intravenously with three types of typhoid vaccine

xvi) Staphylococcus mycolysis as an antigen in rabbits

xvii) Experiment with mycolysin investigating the effect of heat and medium

xviii) Experiments with old mycolysis on coli

xix) Effect of acetone extraction on solution by mycolysin and trypsin

xx) Intravenous injections into rabbits with acetone extracted vaccine, acetone extracted vaccine mycolysate, and acetone extracted vaccine trypsin digest and living typhoid H

xxi) Absorption anti-typhoid serum with typhoid bacilli and trpysin digest

xxii) Absorption agglutination O with trypsin digest (typhoid)

xxiii) Notes from an article on mycolsyates A. Gratia and J. Alexander (Society Belgian Biologist) CR Soc Biology 1288 1931

Section three: 26 June-4^{Oct. 1931.}

ff. 72-94. Bacterial Antagonism

i) Notes on article on ‘Antagonism’ by Hiss, Zanssen and Russell

ii) Experiment testing for antagonism

iii) Experiment testing staphylococcus inhibitions using cholera, pyocyaneus and prodigiosin, includes a diagram of the plate and how he used the word ‘STAPH’ to illustrate the experiment

iv) Experiment examining whether pneumo inhibits staphylococcus

v) Experiments examining the reaction of staphylococcus and parainfluenza on agar, includes diagram of results after 48 hours July

vi) Experiment examining the lysis of B. coli by old cultures

- vii) Work relating to streptococcus, staphylococcus inhibitor, with detailed notes and diagram

viii) Experiments with double inhibition using (AF) mould – using staphylococcus and prodigiosin]. Results includes coloured diagrams of the results

ix) Inhibitions on plates from patients, includes diagrams of plates

a. Page – tooth socket

b. Meynick – post nasal swab

Link with other notebooks:

56144 and 56145: Acetone extracted vaccine

56162: New moulds

56164: Phage

56167: Inhibition

56169: Sample from Myenick

includes:

• ff. 2-5 Pharmacology antiseptics: Experiments by A. Fleming rel. to monsol: 1927-1931.
• ff. 7, 58, 65 Immunology: Experiments and notes by A. Fleming rel. to vaccines: 1902-1948, n.d.
• f. 10 Chemical process: Experiments by A. Fleming rel. to filtration: n.d.
• ff. 15, 17-68 Andre Gratia, Professor, Institute of Bacteriology, University of Liege: Notes and experiments by A. Fleming rel. to Gratia's work: 1930-1931, n.d.
• ff. 17, 41-42 Bacteriology: Experiments by A. Fleming rel. to streptothrix: 1930-1948.
• ff. 17-68 Biochemistry: Experiments by A. Fleming rel. to trypsin: 1917-1932, n.d.
• ff. 36, 43, 59, 62-65, 68 Biology: Experiments by A. Fleming rel. to mycology: 1930-1948.
• ff. 51, 55, 56-57 Scientific method: Patient notes, including graphs, taken by A. Fleming: 1907-1948, n.d.
• ff. 58, 65-67 Pathology infectious diseases: Experiments by A. Fleming rel. to typhoid and paratyphoid: 1908-1949, n.d.
• f. 67 Haematology: Experiments by A. Fleming rel. to agglutination: 1902-1936, n.d.
• ff. 72-94 Biochemistry: Experiments by A. Fleming rel. to antagonism: 1931.
• ff. 72-94 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.