Add MS 56169

FLEMING PAPERS. Vol. LXIV. 1 Jan.-28 Nov. 1932.

ff. ii + 214. 326 x 204mm. Charles Lett’s ‘self-opening’ diary for 1932, with a hard, cream cover with red binding.

Many pages in this notebook have been cut out.

Notebook includes:

Section one:

ff. 1, 3. Headed paper from the British Medical Association Centenary Meeting, London. One with notes and figures on the back relating to experiments with tears and eggs. The other with figures relating to an experiment with range of bacteria and different organs and a turnip; n.d.

Section two: 1 Jan. 1932.

ff. 24-25. Antiseptics

i) Definition of antiseptics

ii) Two problems with the use of antiseptics – inside and outside the body

Outside

a. Sterilisation of instruments after washing

b. Sterilisation of infection ‘excreta’

c. Use as mouthwashes, toothpastes etc

Inside

a. Unified strength

b. Specifity

c. Time of action

d. Affinity for tissues

e. Bacteriostatic action

f. Indirect action

g. Interference by fluids and tissues

iii) Standard tests – slide cells, worms and modification

iv) List of new antiseptics

Section three:

1. f. 31v. Experiments with new mould 1 and 2 and a list of bacteria grown on boiled blood agar plates; 14 Jan.

2. f. 32. Details of samples taken from Montgomery and Dunlop; 15 Jan.

3. f. 33v. Results of samples from Nellie, Sanders and Milbourne; 18 Jan.

4. f. 34. Experiments with new moulds and P. notatum streaked across plates; 19 Jan.

5. f. 35. Tests on new ‘green’ mould titration of growth in test tubes including diagram of agar plate; 21 Jan.

6. ff. 37v-38. Notes on new moulds and penicillin; 26-27^Jan.

7. f. 40v. Experiments with tellurite; 1 Feb.

8. f. 45v. Experiment using AF blood taken from a finger using a paraffined tube and then centrifuged.

9. f. 47v. Tellurite on haemolytic streptococcus – Byrne, Marcus Johnson and Cross; 15 Feb.

10. f. 48. Tellurite on coli – Hall, Bratt, Prior and Rossdale; 16 Feb.

11. f. 48v. Experiments with mould no 19; 17 Feb.

12. ff. 51-52v. Work on Charlottes Pfeiffer’s with samples taken from Clack, Webster, Marchand, Barsdall and Rawson; 22-25 Feb.

Section four: 27^{Feb.-3 March.}

f. 53v-56. Experiments with Precipitation

i) Typhoid rabbit I serum

ii) Centrifuged versus inoculation

iii) Mycolysates

Section five:

1. f. 62v. Experiments with ‘Novaseptic’ (received that day from Mr Scholl of Pharmaceutical [Corporation] Ltd); 16 March.

2. f. 64v. Experiment using rabbit precipitations and serum from 15 March.

3. f. 71. Experiment with old penicillin; 31 March.

4. f. 91. Experiment using lysozyme in eggs crossed through.

5. f. 95. Inhibition shown by penicillin (Meynick) includes diagrams; 10 May.

Section six: 30^{May-18 July.}

ff 105-107, 114v-115v, 117v-119, 121, 124, 126-128, 129v-130v. Tellurite

i) Inhibitions in broth

ii) Experiment with an agar plate half covered with tellurite, includes diagram of results

iii) Dilutions made in bulk

iv) Inhibitions tested against various bacteria

v) Tellurite mould

vi) Mucoid colonies

vii) Resistance coli

Section seven:

1. f. 157v. Experiments with old lysozyme; 12 Sept.

2. f. 160. Experiment using a penicillin and tellurite combination; 17 Sept.

3. f. 164v. Experiment with trypsin lysozyme and phage permanently in agar; 26 Sept.

4. f. 182v. Dyes as inhibitors, tested using diffusions in agar with a range of bacteria; 10 Oct.

5. ff. 188v-189. Tartar emetic as a selective agent; 24-25^Oct.

6. f. 194v. Experiments with ‘neutropsin’ including a note taken from advertising literature that this inhibits B. coli much more than typhoid paratyphoid dysentery; 5 Nov.

7. ff. 209v-210. 27-28 Dec.

List of names, bacteria (including where from) and figures relating to experiments with rabbit haemoglobin

i) Hoad

ii) Read

iii) Smart

iv) Dean

v) Sandford

vi) Lawrence

vii) Brewer

viii) Corrick

ix) Pegler

x) Wootten

xi) Bewley

xii) Couzen

xiii) Taylor

xiv) Carter

xv) Shelier

xvi) Mille

xvii) Harsell

8. ff. 211v-213. 29^{Dec. 1932-1 Jan.1933.}

List of names and notes on location of sample and a description of the bacteria it produced

i) Bewley

ii) Betty Couzen

iii) Miller

iv) Carter

v) Hiller

vi) Hill

vii) Tayler

viii) Barry

ix) Pegler

x) Brewer

xi) Corrick

xii) Lawrence

xiii) Read

xiv) Dean

xv) Sandford

xvi) Hoad

xvii) Smart

xviii) Wootten

xix) Taylor

Links with other notebooks:

56160 and 56171: Tellurite (although dates differ)

56162: Penicillin and moulds

56168: Sample from Myenick

56169: Novaseptic

includes:

• ff. 24-25 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• ff. 31v, 34-35, 37v-38, 48v Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• ff. 31v, 51-52v, 209v-213 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.
• ff. 32-33v, 209v-213 Scientific method: Analysis of samples from patients taken by A, Fleming, some of which include a description of their complaints: 1929-1944, n.d.
• ff. 34, 37v-38, 71, 95, 160 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 40v, 47v-48, 105-107, 114v-115v, 117v-119, 121, 124, 126-128, 129v-130v, 160 Pharmacology antiseptics: Experiments by A. Fleming rel. to tellurite: 1932-1937, n.d.
• f. 45v Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• f. 47v Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• ff. 53v-56, 64v Chemical process: Experiments by A. Fleming rel. to precipitation: 1932-1940.
• f. 62v Pharmacology antiseptics: Experiments by A. Fleming rel. to 'novaseptic': 1932.
• f. 91 Biochemistry: Experiments and notes by A. Fleming rel. to eggs: 1909-1948.
• ff. 91, 157v, 164v Biochemistry: Experiments and notes by A. Fleming rel. to lysozyme: 1921-1948, n.d.
• ff. 95, 194v Chemical process: Experiments by A. Fleming rel. to inhibition: 1917-1942.
• f. 164 Biochemistry: Experiments by A. Fleming rel. to trypsin: 1917-1932, n.d.
• f. 182v Chemical compounds: Experiments by A. Fleming using dyes: 1926-1932.