Add MS 56173

FLEMING PAPERS. Vol. LXVIII. 1 Jan. 1937-3 May 1940.

ff. iii + 372. 321 x 198mm. Hard red cover with red, leather binding. Inside the front cover is a label for ‘W. Isacke, Manufacturing Stationer and Printer, 133 Praed St., London.’

Notebook contains many experiments related to sulphonamides, in particular 693; this work could have formed the basis of two articles Fleming published in 1939 and 1940.

Notebook also contains many loose sheets and photographs of slide cells.

Notebook includes:

Section one:

1. ff.1-19. Requests for examination of specimens and other work for Harefield Hospital (all signed by A. R. Sandford)

i) Casselden

ii) Skinner

iii) Burge

Section two:

1. ff. 24-35. Contents list

Section three: 1-5^{Jan. 1937.}

ff. 38-41. Sulphonamide

i) Absorption

ii) On pneumococcus

iii) Inhibition on plates, including diagrams

iv) ‘Absorption’ by staphylococcus

Section four: 13-17 Feb. 1937.

ff. 42-43. Tellurite

i) On slide cells

ii) On entercoccus ‘Young’

Section five: 3 April 1937.

ff. 44-46. Acelaldehyde

i) On staphylococcus and Hay

ii) On Hay Bacillus spores

iii) On thick Hay bacteria in suspension (2 drops)

Section six:

1. f. 47. Is penicillin dialysed through cellophane? Results include a coloured diagram of a plate; 28 April 1937.

2. f. 48. Staph anti toxin in agar; 8 April 1937.

3. f. 49. Cholera on cellophane; 11 May 1937.

4. f. 50. Recovery of chronogenic properties on paper using prodigiosin and B. violaceus; 14 May 1937.

5. f. 52. Notes from ‘germicidal evaluation of disinfectants’ by Hunt in Journal of Infectious Diseases 1937, 60, 232; n.d.

Section seven: n.d.

ff. 53-54, 57-63, 66. Staphylococcus

i) On antitoxin agar

ii) On normal serum agar

iii) On boiled blood agar

iv) On agar containing boiled serum

Section eight:

1. ff. 70-75. Staphylococcus on agar plates containing BW & Co staphylococcus antitoxin; 17-20 July 1937.

2. ff. 80, 82-87. BW& Co antitoxin; 23 July.

3. f. 81. Toxin and antitoxin in agar; 26 July 1937.

4. f. 94. Longevity of cholera cultures; 1 Oct.1937.

5. ff. 92v-95. Haemolysis human blood agar; 3 Oct. 1937.

6. f. 96. McElligott’s sulphonamide plates; 25 Nov. 1937.

7. f. 98. Notes from an article on ‘Potassium Tellurite as an Indictor of Microbial Life’ by W. E. King and L. Davis in American Journal of Public Health, 1914, 4, 917.

8. f. 99. Notes from an articles on ‘Tellurite Reaction with Coli Typhoid and other Organisms’ 1914 by L. Davis in Centralblatt für Bakteriologie, p.180; n.d.

Section nine: 1 Dec. 1937-21 Jan. 1938.

ff. 100-104, 115. Coagulation

i) Effect of implants on coagulation of plasma – implants into plasma from 24 hour broth culture and two day old capillary tube serum culture

ii) Coagulase staphylococcus

iii) Staphylococcus haemolysis on blood agar

iv) Staphylococcus coagulase

v) Comparison of living and boiled staphylococcus broth in coagulase

vi) Staphs coagulase

Section ten:

1. f. 116. Dialysability of haemolysin – Richards streptococcus, staphylococcus and AEW sarcina (includes diagrams); 21 Jan. 1938.

2. ff. 119-124. Staphylococcus and hemidisc on cellophane on human blood agar, includes photographs of two plates showing that staphylococcus haemolysis is incapable of passing through cellophane and four photographs showing the reaction of selected staphylococcus on human blood agar after 24 hours, one day, three days and fours days; 24 Jan. 1938.

3. f. 125. Sulphonamide on AEW sarcina; 25 Jan. 1938.

4. ff. 127-129. Exeter bloods, agglutination to typhoid (Oxford emulsion); 6 Feb. 1938. Includes a letter from Medical Supervisor Exeter City Mental Hospital to Fleming, dated 31 Jan. 1939.

5. f. 131. Penicillin – acquired resistance of staphylococcus; 11 Feb. 1938.

6. f. 140. Old P. notatum cultures planted in broth to see if any good; 21 Feb. 1938.

7. f. 143. Experiment to see if staphylococcus lysine will pass though gradocoal membranes. Includes photographs showing haemolysis does not pass through, even after two days growth; 28 Feb. 1938.

8. ff. 156, 161. Acne staphylococcus on human serum agar; 8 March 1938.

9. ff. 157-159. Staphylococcus (acne) on B/W and Co serum agar and acne staphylococcus on human blood agar; 10 March 1938.

10. ff. 175, 179. Kiton fast green (said to influence streptococcus infection). Tests with broth, agar, glucose broth and Richard streptococcus and slide cells and Richard streptococcus; 1 April-9 May 1938.

11. ff. 176-177. Sulphonamides on streptococcus (Richards) in slide cell; 4 May 1938.

12. f. 183. Is very young staphylococcus more resistant to bactericidal power of blood than older cultures? n.d.

Section eleven: 14^{May-27 June 1938.}

ff. 184-233. T693 – Part One

i) On pneumo

ii) On staphylococcus

iii) On pneumococcus in slide cells

iv) On staphylococcus in slide cells

v) On Richards streptococcus in slide cells

vi) On streptococcus pneumo in slide cells

vii) Richard streptococcus and defibrinated blood

viii) On Richards streptococcus in slide cells

ix) Pneumo type I in slide cells with immune system

x) Comparison with sulphonamide on pneumo and streptococcus in blood

xi) Patients serum and blood on streptococcus

xii) Patient and AF blood on Richards streptococcus, includes five photographs of slide cells containing various solutions of blood and serum

xiii) Breaking up slide cells and type 3 pneumo

xiv) Decapsulation of pneumo by drug

xv) Testing three patients taking T639

Section twelve: 27^{June-30 July 1938.}

ff. 235, 329. Titration.

i) Endocarditis II serum, includes photograph of slide cells containing AF blood, endo and saliva

ii) Endo I serum with Richards streptococcus, includes photograph of slide cells containing deleucocyted blood, serum, endo and T693

Section thirteen: 2^{July-14 Sept. 1938.}

ff. 240-278, 292-294. T693 – Part Two

i) Serum of T693 patient (endo I) on various organisms and killing of endo I serum

ii) On various organisms in slide cells

iii) Antibacterial action of various T693 serums, includes photograph of slide cells containing AF, endo and field which have been incubated at 37˚C for 6 hours and left on a bench for 36 hours

iv) Titration patients serums with Richards, includes photograph of slide cells containing endo and field in varying dilutions.

v) Titration of V. D. patients taking T693, includes four photographs of slide cells containing AF defibrinated blood

vi) Titration of serum of T693 patients (Richards streptococcus), includes three photographs of slide cells containing heated and AF deleucocyted blood

vii) On various types of pneumococci

viii) On different types of cocci

ix) Sensitivity of Dyson pneumo to T693

x) On staphylococcus in serum

xi) On different pneumos

Section fourteen:

1. ff. 279-291. Hodges and Mills serum on Richards and pneumo; 21 Sept. 1938.

2. f. 295. Cokhinnis new compound on staphylococcus and streptococcus; 14 Oct. 1938.

Section fifteen: 17 Oct.–11 Nov. 1938.

ff. 296-300. Typhoid and 693

i) On Hunt bactericidals

ii) 693 on Hunt typhoid

iii) Agglutinations - centrifuged versus inoculated

iv) Hunt typhoid bactericidals with and without 693

Section sixteen: 16 Nov. 1938-26 Jan. 1939.

ff. 301-316. T693 –Part three

i) Staphylococcus bactericidals with and without 693

ii) 693 on haemolytic enterococcus

iii) Tolerance of staphylococcus to 693

iv) Hypertonic salt and 693

v) 693 serum and staphylococcus

vi) 693 patients serum on staphylococcus and streptococcus

vii) 693 patients blood

viii) 693 on coli, meningo and septothrix

Section seventeen:

1. f. 317. Slide cells of Macleans tolerant pneumo; 27 Jan. 1939.

2. f. 318. Antiseptics on pneumo in AF blood; 10 Feb. 1939.

3. f. 319. ‘Absorption’ of 693 and sulphonamides with Richard, staphylococcus and entercoccus; 16 Feb. 1939.

4. f. 320. 693 and sulphonamides on staphylococcus in broth; 21 Feb. 1939.

5. f. 321. 693 and carbolic or NaCl hypertonic; 3 March 1939.

6. f. 322. Morley’s spirochetes from pleural effusion; 10 March 1939.

7. f. 323. New guinea pneumo sensitivity to 693; 18 March 1939.

8. f. 324. Chlorazol fast pink (Huggett) on pneumo in slide cells; 31 March 1939.

9. f. 325. Carbuncle treated with 693 versus AF in slide cells tested with ‘Ellis’ staphylococcus; 19 March 1939.

10. f. 328. AEW supernatant; 31 May 1939.

11. ff. 329-330. Fildes caseui medium; 7-10 June 1939.

12. ff. 332, 334, 336. Peptone and sulphonamides; 21-24 June 1939.

13. ff. 337-341. Sulphonamides treated with Richards streptococcus; 13-20 July 1939.

14. f. 343. Penicillin production on different medias; 15 Dec. 1939.

15. f. 344. Testing Buttle and AS 21 against sulphonamides and 693 on Richards streptococcus in slide cells; 29 Jan. 1940.

16. f. 346. Testing AS21 and Buttles stuff on Richards; 1 Feb. 1940.

17. f. 349. AS21 Buttle on staphylococcus; 15 Feb. 1940.

18. f. 351. Flavine on leucocytes; 21 Feb. 1940.

19. f. 352. Staphylococcus coagulase; 26 April 1940.

20. f. 353. Impregnated agar strip method for testing sensitivity of bacteria to chemicals; 3 May 1940.

21. f. iii. Notes on AS15, 16 and 17; n.d.

Links with other notebooks

56172: Sulphonamides (although dates differ, probably more a continuation of work)

56224: Acne

includes:

• ff. 1-19 Harefield Hospital, Middlesex: Requests for specimen examinations and other work, all signed by A. R. Sandford: n.d.
• ff. 38-41, 96, 125, 176-177, 319-320, 332, 334, 336-341, 344 Pharmacology: Experiments by A. Fleming rel. to sulphonamides: 1936-1942.
• ff. 42-43, 98-99 Pharmacology antiseptics: Experiments by A. Fleming rel. to tellurite: 1932-1937, n.d.
• ff. 44-46 Chemical compounds: Experiments by A. Fleming rel. to acelaldehyde: 1937.
• ff. 47, 131, 140, 343 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 48, 53-54, 57-63, 66, 70-75, 119-124, 131, 143, 156, 161, 183, 295, 319-320, 325, 347, 352 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• ff. 49, 94 Pathology infectious diseases: Experiments by A. Fleming rel. to cholera: 1937-1948.
• ff. 50, 322, 346, 353 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.
• ff. 92v-95, 116, 119-124, 127-129, 319, 126-128, 319 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• f. 97 American Journal of Public Health: Notes by A. Fleming from an article by W. E. King and L. Davis entitled 'Potassium Tellurite as an Indicator of Microbial Life': n.d.
• ff. 100-104, 115, 352 Haematology: Experiments and notes by A. Fleming rel. to coagulation: 1938-1940, n.d.
• ff. 156-159, 161 Pathology dermatological diseases: Experiments and notes by A. Fleming rel. to acne: 1910-1943.
• ff. 175-177, 179, 295, 337-341, 344 Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• ff. 184-233, 240-278, 292-294, 296-316, 319, 325 Pharmacology: Experiments by A. Fleming rel. to T693 and 693: 1938-1940.
• ff. 235, 329 Chemical process: Experiments by A. Fleming rel. to titration: 1938-1944.
• ff. 279-291, 317-318, 323-324 Bacteriology: Experiments by A. Fleming rel. to pneumococci: 1902-1939.
• ff. 296-300 Pathology infectious diseases: Experiments by A. Fleming rel. to typhoid and paratyphoid: 1908-1949, n.d.
• f. 320 Physiology: Experiments by A. Fleming rel. to absorption: 1927-1939.
• ff. 321, 351 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• f. 351 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.