Add MS 56174

FLEMING PAPERS. Vol. LXIX. 6 May 1940-22 July 1942.

ff. iii + 264. 323 x 195mm. Hard red cover with red, leather binding. Inside the front cover is a label for ‘W. Isacke, Manufacturing Stationer and Printer, 133 Praed St., London.’

Notebook contains many experiments related to sulphonamides; this work could have formed the basis of articles Fleming published in 1940.

Notebook also contains many loose sheets and photographs of plates.

Notebook includes:

Section one:

1. f. 1. Envelope addressed to Fleming at E. M. S. Pathological Laboratory, Harefield Sanatorium. Notes on the back relating to experiment with penicillin versus sulphonamides, n.d.

2. f. 3. Patient and treatment history for James Butler, typewritten on St Mary’s Hospital headed notepaper; n.d.

3. ff. 29-40. Contents list.

Section two: 6-7 May 1940.

ff. 41-42. Ciba

i) On staphylococcus

ii) Staphylococcus vaccine (tested on patient ‘Bearly’)

Section three: 6 May-1 June 1940

ff. 43-57. Sulphonamides, 693 and 760

i) Comparisons inhibition of sulphonamides in agar, using impregnated strip method

ii) Sulphonamides in agar – impregnated strip method (includes diagrams)

iii) 693 versus 760 on streptococcus pyocyaneus in slide cells

iv) 693 versus 760 on AEW bugs

v) 760 in carbuncle patient (face)

vi) 760 on staphylococcus in broth

vii) 760 carbuncle serum and Payne

viii) 760 patient’s serum

ix) 760 versus 693 on staphylococcus in serum and in agar

Section three:

1. ff. 58-59. Staphylococcus precipitations in serum; 2 June 1940.

2. f. 60. Pyocyaneus on plates; 10 June 1940.

3. ff. 62-64. 760 patients serum and 760 pyocyaneus in plates; 12 June 1940.

4. ff. 67v-68. Staphylococcus in serum agar (planted in spots) includes photos of colonies produced; 15 June 1940.

5. f. 70. 760 versus 693 on pneumo – impregnated strips in blood agar; 5 Sept. 1940.

6. ff. 71-72, 74. Instructions and diagrams to facilitate penicillin extraction; n.d.

7. f. 75. Piece of plain paper with notes relating to penicillin – includes reference to an article by Clutterbuck and Raistrick in Biology Journal 26, 1932.

8. f. 76. Experiment with coagulase, strep and transfusions plasma; n.d.

9. f. 77. Experiment using Welchii in milk (litmus milk); 13 July 1940.

10. f. 78. List of bacteria received from National Collections; 18 July 1940.

11. f. 79. Chemicals on anaerobes on plates; 15 July 1940.

Section four: 15 Aug. 1940.

ff. 82v-83. Harefield Hospital

i) Experiments with streptococcus and 693

ii) Experiment with resistant streptococcus (marked as ‘IMPORTANT’)

Section five:

1. f. 84. Pus fluid on 693; 30 Aug. 1940.

2. ff. 85-86. Penicillin versus sulphonamide in slide cells; 4 Sept. 1940.

3. f. 87. Sulphathiazole patients serum; 4 Oct. 1940.

Section six: 6-30 Sept. 1940.

ff. 88-93, 94v-128. Penicillin

i) In broth

ii) On staphylococcus in broth

iii) On staphylococcus in agar

iv) Acclimatisation of staphylococcus to penicillin

v) Development of penicillin in old cultures

vi) Titration of Oxford Penicillin

vii) Intravenous injection penicillin in rabbit

viii) Titration penicillin and establishment of fastness

ix) Comparison penicillin with 693 and 760 in holes in agar

x) Comparison sulphonamide 693 and 760 in holes in agar

Section seven:

1. ff. 129-130. Experiments examining the rate of killing of organisms in boiling water; 31 Oct.-2^{Nov. 1940.}

2. f. 131. Sterilisation of Spencer Wells (blade) in boiling water after being very heavily infected with pus; 5 Nov. 1940.

3. f. 136. Titration penicillin on synthetic medium; 20 Dec. 1940.

4. f. 138. Penicillin on anaerobes; 4 Jan. 1941.

5. ff. 141-142. Notes from articles, n.d.

i) Colin M. McLeod, ‘Sulphonamide Inhibitors’, Journal Experimental Medicine, 1940, 72, 217

ii) E. P. Abraham and E. Chain, ‘Penicillinase’, Nature, 1940, 146, 837.

iii) MacLeod, ‘Pus inhibits Sulphonamide’, Journal of Experimental Medicine, 72, 217

iv) N. Gardner, ‘Morphological affects of Penicillin’

6. f. 150. Oxford penicillin versus PD & Co; 7 March 1941.

7. f. 151. Penicillin, 760, 693 and sulphonamide on staphylococcus in serum; 11 March 1941.

8. f. 151v. Production of colours by different cultures of penicillin mould; n.d.

9. f. 152v. Sterilisation of rough gloves; 15 March 1941.

10. f. 154. Bulb pipette testing for contamination; 27 July 1941.

11. f. 155. Capsule staining; 11 Aug. 1941.

12. ff. 156-157. Staphylococcus rings in serum agar; 16-20 Aug. 1941.

13. ff. 158-159. Aquasan on Richards streptococcus in saliva and on staphylococcus in slide cells; 9 Sept. 1941.

14. f. 160. Testing sensitivity of streptococcus to sulphonamides, impregnated strips (similar test with de-leucocyted blood); 29 Oct. 1941.

15. f. 161. 693 and chewing gum – tested on patients with sore throats; 13 Nov. 1941.

16. f. 162. Sulphonamide resistant streptococcus; 15 Nov. 1941.

17. f. 166. Pamino benzioac acid on sulphonamide and penicillin (incorporated into agar); 25 Nov. 1941.

18. f. 171. Penicillin – tight titration; 10 Jan. 1942.

19. f. 172. Bulk penicillin; 15 Jan. 1942.

20. f. 175. Notes on relating to defibrinated blood, inhibitory clotting, anthrax, proteus and liquoid and an article by T. von Haibler and A. A. Miles in Journal of Pathology and Bacteriology 1938, 46, 245.

21. f. 180. Liquoid on bactericidal power of serum to typhoid and on leucocytes; 24 Feb.-3 March 1942.

22. f. 181. Gentian violet on staphylococcus, streptococcus mixture; 28 Feb.-24^{March 1942.}

23. f. 183. Penicillin in broth and peptone in water; 3 March 1942.

24. ff. 184-188. Penicillin grown on apex dox plates – testing by streaks; 7 March 1942.

24. f. 189.Gentian violet isolating streptococcus; 11 March 1942.

25. f. 191. Gentian violet on staphylococcus, streptococcus mixtures on plates; 11 March 1942.

26. ff. 195-203. Rate of development of penicillin on agar; 11 March 1942.

27. ff. 205-207. Testing sulphonamides resistant to streptococcus; 17 March 1942.

28. f. 211. MgS04 and AuS04 on leucocytes; 7 April 1942.

29. ff. 218v-219v. Aspergillus clavatus versus P. notatum; 19 May 1942.

30. f. 211. Penicillin 19 versus 13; 31 March 1942.

31. ff. 212v-213. Experiments with ‘Slatey’ mould, includes a diagram of a plate; 30 April-5 May 1942.

32. f. 235. Typewritten notes on relating to the problems in chemotherapy of wounds; n.d.

33. f. 236. Photograph of a laboratory with an unidentified man; n.d.

34. ff. 242-244. Three pages of typewritten notes on the arrangements for the teaching of pathology, bacteriology, chemical pathology and clinical pathology (possibly at St. Mary’s); n.d.

35. ff. 246-249. Four pages of typewritten notes on the technique of the Vi agglutination test; n.d.

36. ff. 253-254, 261-263. Two copies of typewritten notes on the infections of war wounds (including air raid casualties) entitled ‘Some questions the answers to which we want to know’. One copy has been annotated; n.d.

Links with other notebooks:

56175: Penicillin and continuation of work on inhibition of staphylococcus

includes:

• ff. 1, 43-57, 85-86, 141-142, 151, 160, 162, 166, 205-207 Pharmacology: Experiments by A. Fleming rel. to sulphonamides: 1936-1942.
• ff. 1, 71-72, 74-75, 88-93, 94v-128, 136, 138, 141, 150-151v, 166, 171-172, 183-188, 195-203, 211, 218-219 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• f. 3 Scientific method: Patient notes, including graphs, taken by A. Fleming: 1907-1948, n.d.
• ff. 41-42, 58-59, 67v-68, 151, 156-159, 181, 191 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• ff. 43-57, 62-64, 70, 151 Pharmacology: Experiments by A. Fleming rel. to 760: 1940-1942.
• ff. 43-57, 70, 84, 151, 161 Pharmacology: Experiments by A. Fleming rel. to T693 and 693: 1938-1940.
• ff. 58-59 Chemical process: Experiments by A. Fleming rel. to precipitation: 1932-1940.
• ff. 58-59, 87, 156-157, 180 Haematology: Experiments by A. Fleming rel. to serum: 1902-1947, n.d.
• ff. 60, 77-79, 15-159, 180, 218-219 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.
• f. 76 Haematology: Experiments and notes by A. Fleming rel. to coagulation: 1938-1940, n.d.
• ff. 76, 158-160, 162, 181, 189, 191, 205-207 Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• ff. 82v-83 Harefield Hospital, Middlesex: Experiments by A. Fleming rel. to samples from the hospital: 1940.
• f. 86 Pharmacology: Experiments by A. Fleming rel. to sulphathiazole: 1940-1942.
• ff. 136, 171 Chemical process: Experiments by A. Fleming rel. to titration: 1938-1944.
• f. 140 Journal of Experimental Medicine: Notes by A. Fleming from an article by Colin M. Mcleod entitled 'Sulphonamide Inhibitors': n.d.
• f. 140 Microbiology: Experiments by A. Fleming rel. to penicillinase: 1943-1944.
• f. 141 Journal of Experimental Medicine: Notes by A. Fleming from an article by MacLeod entitled 'Pus Inhibits Sulphonamide': n.d.
• f. 141 Sir Ernst Boris Chain, Kt.; biochemist: Sir Edward Penley Abraham, Kt.; biochemist: Notes by A. Fleming from an article entitled 'Penicillinase' published in 'Nature' by Sir Ernst Boris Chain and Sir Edward Penley Abraham: n.d.
• f. 154 Scientific equipment: Experiments by A. Fleming rel. to pipettes: 1902-1941.
• f. 175 Bacteriology Proteus Vulgaris: Notes by A. Fleming: 1944-1953, n.d.
• f. 175 Pathology infectious diseases: Experiments by A. Fleming rel. to anthrax: n.d.
• f. 175 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• f. 180 Pathology infectious diseases: Experiments by A. Fleming rel. to typhoid and paratyphoid: 1908-1949, n.d.
• ff. 180, 211 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 181, 189, 191 Pharmacology antiseptics: Experiments by A. Fleming rel. to gentian violet: 1927-1942.
• ff. 212-213 Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• f. 235 Pharmacology: Experiments by A. Fleming rel. to chemotherapy: n.d.
• ff. 242-244 London; Secular Institutions. HOSPITALS AND CHARITABLE INSTITUTIONS. St. Mary's Hospital: Notes on the arrangements for the teaching of pathology, bacteriology, chemical pathology and clinical pathology: n.d.: Typewritten.
• ff. 246-249 Haematology: Experiments by A. Fleming rel. to agglutination: 1902-1936, n.d.
• ff. 253-254, 261-262 Pathology: Notes by A. Fleming on the infections of war wounds entitled 'Some questions the answers to which we want to know': n.d.: Two typewritten copies.