Add MS 56175

FLEMING PAPERS. Vol. LXX. 20 July 1942-5 Dec.1947.

ff. iii + 425. 324 x 194mm. Hard green cover with red binding. Inside the front cover is a label for ‘Geo. Pulman & Sons, Ltd., 24 Thayer Street, London, W.1’.

Some of the notes in the notebook may not be in Fleming’s hand.

Notebook contains experiments on sulphonamides; this work could be related to an article published in 1940.

Notebook contains many loose sheets and photographs.

Notebook includes:

Section one: 22 Feb.-4^{Nov. 1944.}

ff. 3-8, 12-19, 21-23. Treatment cards for named patients.

i) resistant gonorrhoea

ii) acute gonorrhoea, untreated

iii) resistant gonorrhoea, 3/1 duration

iv) gente gonorrhoea untreated

v) resistant gonorrhoea

vi) resistant gonorrhoea

vii) resistant gonorrhoea

viii) acute gonorrhoea

ix) resistant gonorrhoea and periurethral abscess

x) resistant gonorrhoea

xi) gonococcal arthritis

xii) resistant gonorrhoea

xiii) resistant gonorrhoea

xiv) acute gonorrhoea

xv) resistant gonorrhoea

xvi) resistant gonorrhoea

xvii) resistant gonorrhoea

Section two:

1. ff. 24-48, 49-77. Two copies of typewritten notes on penicillin cases. Information includes name, age, condition and treatment and total amount of penicillin used. One of the copies also includes three graphs; n.d.

2. ff. 90-91. Typewritten notes on ‘Practical Bacteriology for Nurses’; n.d.

3. ff. 108-112. Pieces of paper stuck into the notebook, with notes on a range of experiments; 20 July-4 Aug.1942.

i) Inhibition by staph

ii) Phagocytosis of KLB

iii) Phagocytosis of KLB with heated serum

iv) Inhibition of ‘inhibitee’

v) Inhibition of ‘inhibitee’ on agar plates

vi) New moulds – staphylococcus streak on original plate

vii) Bacterial inhibition

viii) Sulphathiazole versus 760

Section three: 7-19 Aug. 1942.

ff. 114-125v, 126v-127v. Lambert

Experiments related to Fleming’s patient Harry Lambert. He was suffering from meningitis. Fleming went against Florey’s advice and gave Lambert an intrathecal injection of penicillin before it had been tested on animals, saving Lambert’s life. This section of work follows through Fleming’s treatment of Lambert, experiments he carried out with his blood, serum etc and a record of his condition over time.

Section four:

1. f. 126. Testing staphylococcus inhibitors; 22 Aug. 1942.

2. f. 127. Old penicillin notatum cultures; 24 Aug.

3. f. 128. Penicillin on different staphylococcus (on agar plate); 26 Aug.

4. f. 130. Method of testing sulphonamide sensitively of streptococcus; 22 Sept. 1942.

5. ff. 129, 132. ‘Nurse Kitten’ patient notes

6. f. 132. Testing notatum (Pen A) - received one gram from Coultard; 19 Nov. 1942.

7. f. 135. Letter from F. D Aspein of the Ministry of Agriculture and Fisheries, dated 17 Nov. 1942, with figures on the back.

8. ff. 136-144. Notes relating to penicillin, not all in A. Fleming’s hand.

9. ff. 156, 153. Patient A9737 with gonococcus arthritis treated by IV TAB protein; 22 Dec. 1942. Not all in A. Fleming’s hand.

10. f. 154. Experiment examining inhibition by staphylococcus; 28 Dec. 1942. Not all in A. Fleming’s hand.

Section five: 29 Dec. 1942-9 Jan. 1943.

ff. 155-165. Eggs

i) Lysozyme in dried eggs

ii) Different dried eggs

iii) Dried eggs in holes ad lysodiekticus

iv) Titration of lysozyme of dried egg, yolk and ‘fluid’ albumen

v) Titration of lysozyme content of dried eggs, also minced fresh egg and dried egg albumen

vi) Compare lysozyme content of various dried egg, minced fresh egg and dried albumen

vii) To estimate the relative lysozyme content of the dried eggs etc by timing the appearance of the lysis

Section six: 15-26 Jan. 1943.

ff. 167-182. Penicillin

i) The effect on a septicaemia staphylococcus in slide cells

ii) Experiment with impregnated strips in glucose agar

iii) P. notatum versus penicillin on staphylococcus includes diagram and description of technique

Section seven:

1. ff. 182v-183. Testing organisms of boys at Downside (Worth) Preparatory School, Poole; n.d. (Correspondence with Farlan and Bradley, see 56113 ff. 119-124).

2. ff. 183-186, 194. Staphylococcus versus diphtheria; 16–25 Aug. 1943.

3. ff. 186v-191. Experiments with penicillin, including photographs.

4. f. 196. Lysozyme on nasal mucus versus antiseptics (for Larymyological lecture); 5 March 1943.

5. ff. 197-201v. Staphylococcus versus diphtheria; 15 March 1943.

6. ff. 202-203v. Extra staphylococcus from noses, includes photographs of plates taped to notebook; 23 March 1943.

7. ff. 210-220. Penicillin on staphylococcus; 8 April 1943.

8. f. 210v. Growth of acne bacillus in glucose broth with and without iron strip; 5 April 1943.

9. f. 220v. Dog bite and hand cauterised, record of tests and treatment; 28 April 1943.

Section eight: 8-29 June 1943.

ff. 221v-226v, 232v, 237. Penicillin

i) Versus sulphistazole

ii) Patient’s blood

iii) Patient’s penicillin in serum, including images slide cells taped to the notebook

iv) In blood

v) Does the amount of implant seriously affect the result with penicillin estimated in blood using Milne streptococcus

Section nine: Various patients

1. f. 237v. Champion: impetigo; 28 June.

2. f. 238. Small ulcers above ankle (no pus); n.d.

3. f. 239. Boils. Never had boils before ‘accident’. Spilt culture on her hand which had a small cut on it and was treated with Lysol. Boils appeared nowhere near the cut; 6 July.

4. f. 239v. Pus squeezed out of dorsum of the foot; 12 July.

5. f. 240. Auxiliary abscess; 16 July.

6. f. 241. Postgraduate patient: emphysema; 16 July.

7. f. 246. Arthritis; 20 July.

8. f. 248. Acute arthritis (sulphar resistant); 27 July.

9. ff. 248v-249. 29^{July-5 Aug. 1943.}

Section ten: 3-5 Aug. 1943.

ff. 260-285. Marfanil

i) With PAB and pus, includes photographs of slide cells taped into the notebook

ii) With prontablin bacteriostasis with and without the pus fluid

iii) Pamino benzoic acid – pus, includes photographs of slide cells taped into the notebook

Section eleven:

1. f. 287v. Titration of penicillin; n.d.

2. f. 301. Penicillin bacteriostasis with and without leucocytes; 7 Aug. 1943.

3. ff. 301v-302v. List of patients, samples taken and resultant bacteria, not in Fleming’s handwriting, n.d.

4. ff. 304-306, 336. Experiments with blood and urine, also nurse’s notes; 17-21 Sept.

5. f. 309. Sensitivity different haemolytic streptococcus in blood to penicillin; 28 Sept. 1943.

6. f. 319v. Resistant to penicillin – filtered; 31 Oct. 1943.

7. ff. 320-320v. Resistant gon; 30 Oct. 1943.

Section twelve: 11^{Nov. 1943-8Feb. 1944.}

ff. 331-334v, 339-340. Penicillinase

i) Experiment for the Medical Research Club

ii) Stray organisms

iii) Twelve stray contaminants from plates planted into broth and grown at room temp for three weeks

Section thirteen:

1. f. 353. Penicillin content of serum after intramuscular injections of penicillin; n.d.

2. f. 354. Test two patients – 7 doses of 15000 units penicillin at 15 intervals; 23 Feb. 1944.

3. f. 355. Test of the best strength of blood in serum blood streptococcus mixtures in capillary tubes; 7 March 1943.

4. ff. 356-359. Penicillin bloods; 15 March 1944.

5. ff. 361v-362v. Gonorrhoea resistance, not in Fleming’s hand; 6-24 April 1944.

6. ff. 364-365, 368-369. Subcutaneous and intramuscular drips given to various patients, not in Fleming’s handwriting; 25 May-13 June 1944.

7. f. 367. Proteus sensitivity to penicillin, tested four strains from Park Prewett; 5 July 1944.

8. f. 370. Duration of penicillin in wounds (plugs soaked in 7000 units of penicillin; 11 May 1944.

Section fourteen: 25 Aug.-14 Sept. 1944.

ff. 372-375. Titration

i) Penicillin in pus

ii) Oxford penicillinase

iii) Penicillin using a standard penicillinase

iv) Retitration of Oxford ase in the presence of penicillin

iv) The effect of varying the inoculation of Milne in the capillary tube titration

Section fifteen:

1. ff. 377-378. Matticks antibiotics – streps planted in blood agar; 27 Feb.-26 March 1945.

2. f. 379. Lysozyme pus dissolving thick growth of lysodeikticus; 14 Jan. 1946.

3. ff. 380-381. Experiments with a staph antibiotic; n.d.

4. ff. 382-385. Experiments with a staph inhibitor; 1 Feb.

5. f. 387. Planted streaks Gratia active coli on agar; 21 March 1946.

6. ff. 388-390. Experiments rel. to microbial antagonism; March 1946.

7. ff. 394v-395. Estimation of penicillin in fluids – serum water, glucose, phenol red and strep and included comparison of methods; 28 Aug. 1946.

8. f. 395v. Testing penicillin in blood serum various methods; 10 Nov. 1946.

Section sixteen: 13^{Nov. 1946-30 Jan. 1947.}

ff. 392, 396-414, 416. Streptomycin

i) Testing anaerobically and aerobically

ii) In test tubes and capillaries

iii) On Milne strep

iv) On Klelsulla 4 in diluted media

v) Different methods of inoculation

vi) Effect of serum

vii) Effect serum of titration of streptomycin

viii) On Klesulla 41 in glucose broth and glucose serum water

ix) In slide cells

x) On Friedlander 3

xi) On blood in slide cells

xii) Testing size of inoculum

xiii) Testing effect in serum using a liquid medium

Section seventeen:

1. ff. 407-408. Two copies of typewritten notes on the ‘Recommendations on the Treatment of Civilian Patients with Penicillin’, both with figures on the back; n.d.

2. f. 415. Bactericidal power of serum to typhoid; 26 Feb. 1947.

3. f. 418. Effect of penicillin on blood clotting; 21 June 1947.

4. f. 419v. Tests on Butterworths books destroyed by mould; 26 Oct. 1947.

5. f. 420. Action of penicillin on clotting time of haemophilic blood; 2 Dec. 1947.

6. f. 421v. Inhibitory bacillus – contamination on plate; 5 Dec. 1947.

7. f. 425. Treatment card – resistant gonorrhoea; 22 June 1944.

8. f. 422. Typewritten notes on the ‘Nitrate Reduction Test’; n.d.

Links with other notebooks:

56174: Penicillin and continuation of work on inhibition of staphylococcus

56176: Loose link between work on titration of penicillin

56181: Patient (resistant gonorrhoea)

56183: Various patients. Also more details on the treatment of Harry Lambert.

56225: Penicillin titration

includes:

• ff. 3-8, 12-19, 21-23, 320-320v, 361v-362v, 424 Pathology venereal diseases: Experiments by A. Fleming rel. to gonorrhoea: 1944.
• ff. 3-8, 12-19, 21-23, 424 Scientific method: Treatment cards for A. Fleming's patients: 1944.
• ff. 24-48, 49-77, 114-125v, 126v-128, 132, 136-144, 167-182, 186v-191, 210-220, 221v-226v, 232v, 237, 287v, 301, 309, 319v, 353, 356-359, 367, 370, 394v-395v, 407-408, 418, 420 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 90-91 Bacteriology: Notes by A. Fleming entitled 'Practical Bacteriology for Nurses': n.d.: Typewritten.
• ff. 108-112 Cell biology: Experiments by A. Fleming rel. to phagocytosis: 1902-1942.
• ff. 108-112 Pharmacology: Experiments by A. Fleming rel. to sulphathiazole: 1940-1942.
• ff. 108-112 Pharmacology: Experiments by A. Fleming rel. to 760: 1940-1942.
• ff. 108-112, 126, 154, 382-385 Chemical process: Experiments by A. Fleming rel. to inhibition: 1917-1942.
• ff. 108-112, 419v Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• ff. 112-123 Pathology infectious diseases: Experiments by A. Fleming rel. to meningitis: 1908-1942.
• ff. 114-125v, 126v-127v Harry Lambert, employee of J and R Fleming (Optical firm): Experiments and notes by A. Fleming rel. to Lambert's treatment for staphylococci meningitis: 1942-1948.
• ff. 114-125v, 126v-127v, 129, 132, 220v, 237v, 238-241, 246, 248-249 Scientific method: Patient notes, including graphs, taken by A. Fleming: 1907-1948, n.d.
• ff. 126, 154, 183v-186, 194, 197-203v, 210, 211-220, 380-381 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• f. 130 Pharmacology: Experiments by A. Fleming rel. to sulphonamides: 1936-1942.
• ff. 130, 309, 355 Bacteriology: Experiments by A. Fleming rel. to streptococcus: 1921-1944, n.d.
• ff. 146, 153 Pathology inflammatory diseases: Experiments and notes by A. Fleming rel. to arthritis: 1942, n.d.
• ff. 155-165 Biochemistry: Experiments and notes by A. Fleming rel. to eggs: 1909-1948.
• f. 179 Worth Downside Preparatory School; Poole, Dorset: Experiments and notes by A. Fleming rel. to diphtheria: n.d.
• ff. 183-186, 197-201 Pathology infectious diseases: Experiments and notes by A. Fleming rel. to diphtheria: 1943-1948.
• f. 196 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• ff. 196, 379 Biochemistry: Experiments and notes by A. Fleming rel. to lysozyme: 1921-1948, n.d.
• f. 210v Pathology dermatological diseases: Experiments and notes by A. Fleming rel. to acne: 1910-1943.
• ff. 260-285 Pharmacology: Experiments by A. Fleming rel. to marfanil: 1943.
• f. 301 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 301v-302v Scientific method: Analysis of samples from patients taken by A, Fleming, some of which include a description of their complaints: 1929-1944, n.d.
• ff. 304-306, 336, 355-359, 418, 420 Haematology: Experiments by A. Fleming rel. to blood: 1902-1947, n.d.
• ff. 331-334v, 339-340, 334-337 Microbiology: Experiments by A. Fleming rel. to penicillinase: 1943-1944.
• f. 367 Bacteriology Proteus Vulgaris: Notes by A. Fleming: 1944-1953, n.d.
• ff. 372-375 Chemical process: Experiments by A. Fleming rel. to titration: 1938-1944.
• ff. 377-378, 380-381 Microbiology: Experiments by A. Fleming rel. to antibiotics: 1945-1948.
• ff. 387, 421v Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.
• ff. 392, 396-414, 416 Microbiology: Experiments by A. Fleming rel. to streptomycin: 1947, n.d.
• f. 415 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• f. 415 Pathology infectious diseases: Experiments by A. Fleming rel. to typhoid and paratyphoid: 1908-1949, n.d.
• f. 415 Haematology: Experiments by A. Fleming rel. to serum: 1902-1947, n.d.