Add MS 56178

FLEMING PAPERS. Vol. LXXIII. 5 Dec. 1947-13 July 1953.

ff. i + 116. 321 x 200mm. Hard green cover with green binding.

Label inside the front cover for ‘Geo. Pulman & Sons, Ltd., 24 Thayer Street, London, W.1’.

A large section of the work contained in this notebook has been devoted to the movement of proteus and flagella; this may have formed the basis of articles published by Fleming and Dr Voureka in 1950.

Notebook includes:

Section one: n.d.

ff. 2-4. Photographs of colonies.

i) Plate with two green, one red and one yellow colony

ii) Close up of a ‘large irregular colony, magnified x6, showing phagocytosis with complete lysis’

iii) Plate with two colonies on it, one is inactive penicillin, the other P. notatum

iv) Two streaks of staphylococcus between mould colonies

Section two:

1. ff. 6-12. Handwritten article or book review about penicillin, written in French by J [Rhatcliff?].

2. f. 14. Photograph of Amelia and an unidentified man; n.d.

3. f. 15. Photograph of two unidentified women and one unidentified man; n.d.

4. f. 22. Notes relating to bacillary contamination of plate showed inhibitory action on staph; 5 Oct. 1947.

5. f. 22v. Testing efficiency of capping machine; 1 Dec. 1947.

6. f. 23. Staphylococcus in serum agar; 5 Dec. 1947.

7. f. 25. Experiment using samples of staphylococci taken from noses of students, 25 Jan. 1948.

8. f. 26. Antibiotic contaminations, includes coloured diagrams of results; 26 Jan.-2 Feb. 1948.

9. ff. 27, 29. Hays antibiotics; 18–20 Feb. 1948.

10. f. 29v. Mycolysin – streptothrix from air on water and boiled typhoid; 1 Oct. 1948.

11. ff. 31-32. Proteus growth in penicillin. Left for 24 hours room temp, includes simple drawings of proteus; 8 Jan. 1949.

12. f. 34. Carbolic and polymyxin on proteus, includes simples diagrams and notes ‘nothing like penicillin’; 6 April 1949.

Section three: 20 Sept.-12^{Nov. 1949.}

ff. 35-73. Movement of proteus

i) Timing revolutions of proteus

ii) Timing revolutions of ‘Walchspring’ proteus

iii) Timing revolutions of proteus with different periods of rest (with Dr Voureka)

iv) Timing flagella after different periods of rest – proteus

v) Timing flagella – no movement of organisms

vi) Timing movement of proteus with different periods of rest

vii) Timing proteus – Wherelers recorder (left for 24 hours)

viii) Timing revolutions of proteus – using wire recorder

ix) Timing proteus – wire recorder, hest filter in while slide examined for suitable ‘Walchspring’

x) Timing flagella – microbes not moving

xi) Timing proteus – wire recorder –spreading for thick streak and spread from streak of solid culture

Section four: 28 Nov.-28 Dec. 1949.

ff. 75-88. Fatigue on proteus

i) ‘Walchspring’ form

ii) Overnight in slide culture

iii) Triple coil ‘Walchspring’

iv) In penicillin culture, 24 hours room temperature and 24 hours in an ice chest

v) 24 hours room temperature

Section five:

1. ff. 89-90. Electrical stimulation of proteus (culture overnight, on agar); 4 Jan. 1940.

Section six: 7 Jan.-6 Feb. 1950.

ff. 93-96. Proteus motility

i) Effect of a heat filter and a ground glass filter

ii) The rhythmic alteration of [sale?] of an organism while ground glass filter

iii) 24 hours in room temperature culture in penicillin agar, noticed ends were working against each other something that he had not seen before

Section seven: 27 April-27 June 1950.

ff. 98-102. Rhythmic movements

i) Circular movements of proteus – clockwise or counter clockwise

ii) Direction of rotation

Section eight:

1. ff. 104-108. Syringe sterilisation; 26-31 July 1950.

2. f. 109. Movement of proteus; 1 Aug. 1951.

3. f. 110. Piece of blotting card advertising ‘Nicorbin’ (tablet containing four vitamins).

4. ff. 112-116. Action of chemical on proteus (Douglas) in broth; 30 June-13 July 1953.

Link with other notebooks:

56183: Proteus

includes:

• ff. 2-4 Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• ff. 2-4, 22 Bacteriology: Experiments, notes and photographs by A. Fleming rel. to bacteria: 1928-1947, n.d.
• ff. 2-4, 22-23, 25 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• ff. 2-4, 6-12, 31-32 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 26-27, 29 Microbiology: Experiments by A. Fleming rel. to antibiotics: 1945-1948.
• f. 29 Biology: Experiments by A. Fleming rel. to mycology: 1930-1948.
• f. 29 Bacteriology: Experiments by A. Fleming rel. to streptothrix: 1930-1948.
• ff. 31-32, 34-73, 75-90, 93-96, 98-102, 104-109, 112-116 Bacteriology Proteus Vulgaris: Notes by A. Fleming: 1944-1953, n.d.
• f. 34 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.