Add MS 56183

FLEMING PAPERS. Vol. LXXVIII. Miscellaneous loose notes, 1925-1948, n.d.

Section one:

1 f. 1. Image of a plate with the autolysis of unleaded pike eggs.

2. f. 2. Note to Fleming from W. Bulloch pointing out an article by O. Bail entitled ‘Ueber das Lysozyme A. Flemings’ in Wiener Klinische Wochenschrift, 1923. XXXVI. 107.

3. ff. 3-4. Fleming’s conclusions about part II of an article by D. W. Weisner entitled ‘Über die Bakterizidie von Leukozytenstoffen, mit besonderer Berückschtigung der [Vashaltisse?] am Auge’, Zeitschrift für Hygiene unde Infektionskrankheiten, 1912, Band 72.

4. ff. 5-6. Notes on the action of leucocytes in different temperatures.

5. ff. 7-8v. Fleming’s views on an article by Dr. F. L. Fowrowti in Beiträge für Chemische Physiologie und Pathologie, Band I, 1901.

6. ff. 9-12. Fleming’s summary of conclusions on an article by Dr. H. Gouradi entitled ‘The Formation of Bactericidal Materials by Autolysis’ in Beiträge für Chemische Physiologie und Pathologie, Band I, 1902.

7. ff. 13-16. Fleming’s notes on an article by Dr. H. Gouradi entitled ‘On the nature of the bactericidal substances originating in autolysis.’

8. ff. 17-20. Notes from various articles

i) Leo F. Rettger & Joel A. Sperry ‘The antiseptic and bactericidal properties of egg white’ Journal of Medical Research, Vol. 26, p55, 1912

ii) Bourdet & Luica, ‘Sur la regeneration du principe actif dans l’autolyse microbienne’, CR Society Biology, 85, 1921, p1095

iii) Pozeroki ‘Action de la temperature sur la bacteriophage’ CR Society Biology, 85, p1011

9. ff. 21-22. Fleming’s note on article P. Laschtonchenko ‘The Bactericidal and Bacterio-inhibitory action of hen egg white’.

10. f. 23. Printed article by L. S. Penrose on the ‘The lysozyme content of saliva in psychotics’ Lancet 1930. Article includes a reference to Fleming’s work.

11. ff. 24-27. Printed article by Marguerite Bordet ‘Contribution to the Study of Lysozyme’ 1928, in Comptes rendves des Seances de la Societe de Biologie, October 1928, XCIC, p1254.

12. f. 28. Review of an article by G. M. Findlay on the ‘Lysozyme content of tears’ in British Journal of Experimental Pathology, VI, 1925, p16. Unknown author.

13. f. 29. Fleming’s notes from an article by S. L. Baker and S. H. [Lanasutty?] on ‘Ultraviolet on Lysozyme’ in British Journal of Experimental Pathology, 1929, X, 45.

14. f. 31. List of references for articles on lysozyme.

15. ff. 32-33. Fleming’s notes on article by Rettger and Sperry on ‘The antiseptic bactericidal properties of egg white’ in Journal of Medical Research, Vol., XXVI, Apr 1912-July 1912, no130, p55.

16. ff. 34-35. Fleming’s notes on article by [Laupe] on the ‘Penetration of bacteria into hen eggs through the shell’, in Archiv Für Hygiene, B.62, S.201, 1907.

17. f. 36. Fleming’s notes on an article by Sachs-Hücke ‘Can dysentery bacilli grow though the wall of the fresh hen’s eggs?’ in Archiv Für Hygiene, B.62, S.229, 1907.

Section two: n.d.

ff. 38-43. Penicillin

i) Graph showing blood levels after 15,000 units were given intravenously, intramuscularly and subcutaneously. Notes on the back relating to haemolytic power of human serum and complementation

ii) Graph showing the effect of 15,000 units of calcium penicillin given intravenously on serum

iii) Graph showing the effect of intravenous injections every two hours on penicillin blood levels

iv) Graph entitled ‘Rapidly repeated doses’, it shows the effect of intramuscular injections of 20000 units every fifteen minutes

v) Graph to show the effect of injections of 100,000 units intramuscularly with 1% procaine on penicillin blood levels

i) Graph to show the effect of 100,000 units intramuscularly of calcium and sodium penicillin on blood levels

Section three: n.d

ff. 46-56. Staphylococcus toxin: Antitoxic response

i) Graph showing the precipitation production from a single injection of horse serum into a) normal rabbit b) rabbit previously sensitised

ii) Graph showing antitoxin production in Horse, showing difference between primary and secondary response

iii) Six pieces of card with notes on:

a. Antitoxic power of blood type before and after toxoid treatment

b. Resistance of antitoxin after treatment

c. Some recurrences with high antitoxic power

d. Results of treatment

e. The number of patients who stopped attending after two or three doses of toxoid (being treated for boils)

f. Illustrative cases

iv) List of patients, a number and notes of the number of doses they received and the amount

ii) Handwritten notes relating to the trial, possibly a draft for a article with I. H. Maclean published in 1937 ‘On the use of staphylococcus antitoxin, toxin, toxoid and vaccine’ for Second International Congress for Microbiology, 25 July -1 August 1936, Report on Proceedings, London, p519

Section four:

1. f. 58. Graph of a patient who was given intramuscular and intravenous injections of penicillin. Graph is figure 7 on f. 58.

2. f. 59. Graph showing summation of intravenous doses (15,000 units every 10 minutes). Does not state who the patient was. On the back note had been attached ‘Figure 3. Bacteriostatic power of patient’s serum after repeated intravenous injections of penicillin at short intervals’. Graph is figure 3 on f. 58.

3. f. 60. Page three of the Lancet, with a number of graphs relating to experiments with penicillin, it is revised copy. Possibly part of a article by Fleming, M. Y. Young, J. Suchet and A. J. E. Rowe on ‘Penicillin content of blood serum after various doses of penicillin by various routes’, Lancet, 2 (1944).

4. f. 61. Two graphs:

i) Patient given 105,000 calcium penicillin intramuscularly

ii) Patient given two doses of 50,000 intramuscularly

5. f. 62. Graph showing the delay of excretion in patient with amyloid kidney. Graph is figure 8 on f. 58.

6. ff. 63-64. Quotes from Lister on the antiseptics taken from various journals, addresses and speeches.

7. ff. 65-66. Notes on antiseptics and a chart showing the effect of the application of Dakins fluid to a wound, noting the rate of exudation of fluid and number of bacteria in fluid.

8. ff. 67-72. Ministry of Health Emergency Medical Service, Sector 6 headed notepaper, with notes on penicillin and antiseptics taken from:

i) British Journal of Experimental Pathology, 1929

ii) Proceedings of Royal Society, 1931 – Intravenous use of germicides

iii) British Dental Journal, 1931

iv) Proceedings of the Royal Society, 1924

v) Proceedings of the Royal Society, 1924

9. f. 73. Handwritten draft of an abstract for Excerpta Medica, n.d.

10. ff. 74-76. Biography of the Louis Pasteur and possibly notes relating to a speech Fleming was to make about Pasteur, including page numbers of a book or article he was using for research.

Section five:

ff. 78-84. Staf v. Diphtheria: Some aspects of vaccine therapy.

This work could have formed the basis for articles on vaccine therapy published in 1939.

i) Handwritten and typewritten results of experiment where broth tubes were infected with one loop full of diphtheria bacillus

ii) Typewritten and handwritten notes on the inhibitory action of various organisms on a sensitive diphtheroid organism

iii) Typewritten description of the experiment

iv) Handwritten and typewritten results of an experiment testing inhibition of different bacteria by the two strains of ‘Inhibitor’ staphylococci

v) Handwritten and typewritten results of experiment with Leoffler’s slopes infected with mixtures of equal volumes of undiluted broth cultures of diphtheria bacillus and dilutions of staphylococci

vi) Handwritten note from Florey to Fleming with notes on relating to the inhibition of staphylococcus

Section six:

1. f. 87. Drawings of normal B. typhosus and it grown in penicillin.

2. ff. 89-93. Article by H. Florey on ‘The use of micro-organisms for therapeutic purposes’ in British Medical Journal, London, Saturday November 10 1945. A loose sheet has been inserted with notes on relating to actinomycin and aureumycin.

3. ff. 99-102, 105. Proteus drawings:

i) Typhoid broth 3 days at room temp; 22 Nov. [1947].

ii) Typhoid after 24 hours in agar at 37˚C and 5cc of penicillin

iii) Typhoid, cholera and proteus in various solutions, 25 Nov. [1947].

iv) Proteus 1/8 agar and 5cc at 48 hours room temperature

v) Cholera 24 hours room temp, 10/ serum and 2.5cc penicillin

vi) Proteus 2.5cc penicillin, 24 hours room temperature

vii) Typhoid 24 hours on radiator with 5cc penicillin; 31 Jan. 1949.

4. ff. 108-111. Notes on various concentrations of plate solutions and descriptions of the effect on proteus, includes diagrams; 30 Nov.-15 Dec. [1949].

5. ff. 113-127. Proteus drawings:

i) 25cc penicillin and 24 hours room temperature, noted ‘motion is intermittent and when motion ceased the movement of flagella could be seen’

ii) 1/8 agar, 5cc, 48 hours room temperature in a pool

iii) 25cc, 24 hours room temperature (broth), drawings at 11.45, 12.30, 1.10 and 2.30

iv) 2.5cc in agar, noted much distortion but CuChlorophyll had no effect on morphology

v) 25cc, 24 hours room temperature, Fleming has noted the direction of the movement, 21 March 1949.

vi) Two drawings and Fleming has noted that the ‘rope not strong enough the rotate the organism’ and the effect of a heat filter on its movement

6. f. 129. Testing the effect of light on the movement of flagella; 5 April 1948.

7. ff. 130-134. Proteus drawings:

i) 20cc, room temperature for 24 hours. Noted movement stops and starts with light; 5 May 1949.

ii) 20cc at room temperature, noted surrounded but not moved; 17 May 1949.

8. ff. 135-138. Notes on effect on proteus over time, includes diagrams.

9. f. 142. Handwritten notes describing the movement of proteus and the effect of light on its movement, includes diagrams.

10. f. 148. Notes on proteus versus penicillin.

Section seven: Folder labelled ‘toxic penicillin’; 10 Feb.-17 May 1944.

1. ff.150-160. Clinical charts

2. ff. 161-165. Notes relating to a letter from W. J. Hurran of Glaxo Laboratories to Fleming dated 10 May 1944, relating to testing samples of penicillin; 24 April-4 May 1944. See 56113, ff. 170-171.

Section eight:

ff. 166-174. Handwritten notes about lysozyme in an unknown hand.

Section nine: Folder labelled ‘Charts showing Penicillin Blood Levels’, [1943].

1. ff. 176-178, 180-184, 186-194. Graphs

i) intramuscular

ii) Pneumo, trypsin and nucleic acid, given intravenously

iii) 17 Nov. 1943.

iv) 100,000 Na salt IM

v) [Named patient]

vi) Graph showing changes to two patients

vii) 20T intramuscularly

viii) intramuscularly

ix) 17 Nov. 1943, with note attached

x) intramuscular

xi) [Named patient]

2. f. 185. Penicillin class timetable for the Inoculation Department lecture theatre.

3. f. 192. Graph with typewritten note on the back ‘Figure 1. Bacteriostatic power of patient’s serum after i) intravenous and ii) intramuscular injection of 15,000 units of penicillin’. This could link with article for the Lancet. See 56183 f. 58.

4. f. 198. Notes of treatment.

5. f. 205. List of things associated with penicillin

i) Preparation

ii) Properties

iii) Titration

iv) Pharmacy

v) General

vi) Local

6. f. 207. Graph, typewritten note on the back reads ‘Figure 2. Bacteriostatic power of patient’s serum after intravenous and intramuscular injections and a continuous intravenous drip at different rates’. This could link with article for the Lancet. See 56183 f. 58.

7. ff. 212-215. Typewritten notes about patient diagnosed with chronic gonococcal arthritis:

i) Medical history

ii) Notes from his admittance to St. Mary’s

iii) Treatment and subsequent progress

iv) Treatment with penicillin

v) Observations on clinical condition of the patient during penicillin treatment

8. ff. 216-218. Typewritten notes about patient with primary syphilis:

i) Previous history

ii) History of present infection

iii) Examination

iv) Investigations

v) Treatment with penicillin

vi) Progress after cessation of penicillin therapy

vii) Summary

Section ten:

1. ff. 220-221, 223-224. List of patients, their condition and the effectiveness of their treatments.

2. f. 222. Letter from Almroth Wright to Fleming relating to patient and his treatment with penicillin cream.

3. f. 228. Notes on the effectiveness of penicillin on treating impetigo and auxiliary abscesses.

4. ff. 230-232. Patient record for Sir Montagu Norman (Governor of the Bank of England), who was diagnosed with pneumococcal meningitis and treated with penicillin; 24-26 Jan. 1944.

5. ff. 233-235. Record for patient with osteomyalitis of the skull.

6. ff. 236-247. Penicillin cases, including clinical charts:

i) D. W: breast abscess

ii) J. G: Orbital cellulites

iii) A. C: Subacute bacterial endocarditia

7. ff. 256-258. Handwritten notes on the preparation of mould colonies for museum purposes. Possibly work towards a lecture for the Linnaean Society of London, later published as ‘The use of paper and cellophane discs for the preparation of museum specimens of mould cultures’ in the Proceedings of the Society, 36 (1943), 5-6.

8. f. 260. Handwritten notes relating to leucocytes and coagulation of the blood.

Section eleven: [7 July-20 Aug. 1942.]

ff. 276-324. Harry Lambert

1. f. 278. Notes on when treatment with penicillin commenced.

2. ff. 279-283. Clinical charts.

3. ff. 284-287, 310. Records of his treatment and diet; 8-20 Aug. 1942.

4. f. 288. Dr. Gregg’s notes on Lambert’s condition, 7 July 1942.

6. ff. 291-293. Charts showing Lambert’s fluid intake and urine output from 9 July–19 Aug. 1942.

7. ff. 289-290, 295-298, 301-308, 312-313. Pathological request forms

i) Blood

ii) Urine

iii) Routine examination

iv) Hl and RBC’s [red blood cells]

v) White count

vi) Complete blood count

vii) Full blood count

viii) DUBC’s, Hl and RBC’s

ix) Agglutination reaction

x) WR

xi) Cells

xii) Organisms

xiii) Blood culture

xiv) Gold curve, protein and WR

xv) Blood urea

xvi) Routine analysis

xvii) BSR

8. f. 299. Letter from G. Roche Lynns to Dr. Gregg, relating to Lambert and crystalline deposit in urine, he thinks it might be leucin but requests a larger sample, dated 5 Aug. 1942.

9. f. 309. Letter to Dr Gregg from Dr F. Crosby of West Ealing, referring to first attack of haemorrhoids and Lambert’s subsequent problems, dated 7 July 1942.

10. f. 311. Radiology request for an x-ray of the chest. Radiologist noted heart was a normal size and lungs were clear, 7 July 1942.

11. f. 314. List of jobs related to his treatment.

12. ff. 315-318. Typewritten notes relating to his treatment, including admittance to hospital, x-rays, tests and fluid and food intake.

13. f. 319. Nurses’ notes.

14. ff. 321-324. Notes on bacteriostatic tests with centrospinal fluid.

Links with notebooks:

56149: Patient.

56175: Patients. Also more details on the treatment of Harry Lambert.

56176: Patients.

56178: Proteus.

includes:

• ff. 2, 23-29, 31, 166-174 Biochemistry: Experiments and notes by A. Fleming rel. to lysozyme: 1921-1948, n.d.
• ff. 5-6, 260 Haematology: Experiments by A. Fleming rel. to leucocytes: 1917-1948, n.d.
• ff. 9-16 Pharmacology: Experiments and notes by A. Fleming rel. to bactericidal: 1902-1947, n.d.
• ff. 17, 32-33 Journal of Medical Research: Notes by A. Fleming from an article by Leo F. Rettger and Joel A. Sperry entitled 'The Antiseptic and Bactericidal Properties of Egg White': n.d.
• ff. 17-22, 32-35, 36 Biochemistry: Experiments and notes by A. Fleming rel. to eggs: 1909-1948.
• ff. 21-22 Biochemistry: Notes by A. Fleming on P. Laschtonchenko's article entitled 'The Bactericidal and Bacterio-inhibitory Action of Hen Egg White': n.d.
• f. 23 Physiology: Notes by A. Fleming on an article rel. to saliva: 1925-1948.
• f. 23 Lionel Sharples Penrose, Professor of Eugenics, University College London: Article entitled 'The Lysozyme Content of Saliva in Psychotics', published in the Lancet: 1930: Printed.
• f. 28 British Journal of Experimental Pathology: Review of an article by G. M. Finlay entitled 'Lysozyme Content of Tears': n.d.
• f. 29 British Journal of Experimental Pathology: Notes by A. Fleming on an article by S. L. Baker and S. H. [Lanasutty] entitled 'Ultraviolet on Lysozyme': n.d.
• ff. 38-43, 58-61, 67-72, 87, 113-127, 148, 161-165, 176-178, 180-194, 205, 207, 222, 228, 230-232, 236-247, 276-324 Microbiology penicillin: Experiments and notes by A. Fleming: 1928-1949, n.d.
• ff. 46-56, 78-84 Bacteriology: Experiments and notes by A. Fleming rel. to staphylococcus: 1910-1947, n.d.
• ff. 54-56, 78-84 Immunology: Experiments and notes by A. Fleming rel. to vaccines: 1902-1948, n.d.
• ff. 56, 198, 212-218, 220-221, 223-224, 230-235 Scientific method: Patient notes, including graphs, taken by A. Fleming: 1907-1948, n.d.
• ff. 63-72 Pharmacology antiseptics: Experiments and notes by A. Fleming: 1916-1949, n.d.
• ff. 74-76 Louis Pasteur, French microbiologist: Notes by A. Fleming, possibly relating to a speech: n.d.
• ff. 77-85 Pathology infectious diseases: Experiments and notes by A. Fleming rel. to diphtheria: 1943-1948.
• ff. 89-93 Sir Howard Walter Florey, Baron Florey: Article entitled 'The Use of Micro-Organisms for Therapeutic Purposes' published in the British Medical Journal: 1945.
• ff. 99-102, 105 Pathology infectious diseases: Experiments by A. Fleming rel. to typhoid and paratyphoid: 1908-1949, n.d.
• ff. 99-102, 105 Pathology infectious diseases: Experiments by A. Fleming rel. to cholera: 1937-1948.
• ff. 99-102, 113-127, 130-138, 142, 148 Bacteriology Proteus Vulgaris: Notes by A. Fleming: 1944-1953, n.d.
• f. 129 Bacteriology Proteus Vulgaris: Notes on flagella by A. Fleming: 1931-[1948]: Partly typewritten.
• ff. 150-160, 236-247 Scientific method: Clinical charts of A. Fleming's patients: n.d.
• ff. 212-215 Pathology inflammatory diseases: Experiments and notes by A. Fleming rel. to arthritis: 1942, n.d.
• ff. 216-218 Pathology venereal diseases: Experiments and notes by A. Fleming rel. to syphilis: n.d.
• ff. 230-232 Montagu Collet Norman, Baron Norman of St Clere; Governor of the Bank of England: Patient record rel. to his treatment for meningitis with penicillin by A. Fleming: 1944.
• ff. 256-258 Microbiology: Experiments by A. Fleming rel. to mould: 1929-1947, n.d.
• f. 260 Haematology: Experiments and notes by A. Fleming rel. to coagulation: 1938-1940, n.d.
• ff. 276-324 Harry Lambert, employee of J and R Fleming (Optical firm): Experiments and notes by A. Fleming rel. to Lambert's treatment for staphylococci meningitis: 1942-1948.